Corresponding Author: Norma Suárez Department of Biotechnology, Institute of Hygiene, Faculty of Medicine University of the Republic. Uruguay Phone: +5982 487 1288 ext 1123. Fax: +5982 487 3073 E-mail: [email protected] Abstract: Streptococcus pneumoniae, a major bacterial pathogen worldwide, requires a complex medium for culture, which complicates harvesting of antigens for vaccine produc...

The gonads of Deontostoma californicum were isolated from the organismal environment by dissection. In an attempt to approximate the in vivo condition and to maintain in vitro architecture and function ten media recommended for the culture of insect cells or organs were tested. The media were prepared in two series: one according to published recommendations and the second substituting filtered...

In vitro production (IVP) systems have been used to bypass problems of fertilization and early embryonic development. However, embryos produced by IVP are commonly selected for implantation based on morphological assessment, which is not a strong indicator of establishment and maintenance of pregnancy. Thus, there is a need to identify additional indicators of embryonic developmental potential....

Researchers have very limited understanding of how continuous usage intention of social media (SM) are associated with online benign envy and individual-level culture. This paper addresses this gap and provides a theoretical framework to illustrate how manifestations of national cultural values at the individual level of analysis may influence SM use behaviors through online envy. We argue that...

Nanoparticle research is often performed in vitro with little emphasis on the potential role of cell culture medium. In this study, gold nanoparticle interactions with cell culture medium and two cancer cell lines (human T-cell leukemia Jurkat and human pancreatic carcinoma PANC1) were investigated. Gold nanoparticles of 10, 25, 50, and 100 nm in diameter at fixed mass concentration were tested...

A technique is described in which continuous culture is used for the optimization of media in terms of growth-supporting ability. The technique consists of identifying growth-limiting nutrients by observing the reaction of the continuous system to injection of suspected growth-limiting nutrients into the growth vessel.

It has been recommended that routine microbiological processing of urine specimens include quantitative plating onto blood agar medium along with a selective and differential agar such as MacConkey agar for gram-negative organisms. Few data have been published to justify this combination. To evaluate the validity of this recommendation 2,553 midstream, clean-voided urine samples were quantitati...

OBJECTIVES To evaluate BD MGITTM TBc® technique for identifying the Mycobacterium tuberculosis complex using isolates obtained in liquid and solid media. METHODS A descriptive study was conducted in which 117 isolates were analyzed by the immune-chromatography technique obtained from solid and liquid cultures to identify the Mycobacterium tuberculosis complex. The kappa coefficient was calcul...