In studies on surface components of virulent mycobacteria, there is a continuous need for relatively large quantities of these organisms as produced in surface culture in Roux bottles. Achieving effective surface growth depends upon adequately seeding the surface of the liquid medium. A practice which may well have been employed even by Koch has been the transfer of a pellicle of "veil growth" ...

stage-specifci developmental forms of lesihmania major promastigotes were grown in vitro. procyclic and metacyclic promastigotes were cultured and separated by peanut agglutinin. the axenic amastigote form were prepared by culturing the promastigotes in acidic medium at 35c.these three forms of parasites were used as antigens to study cytokine production in whole blood culture of healthy indivi...

Embryonic stem cells (ESCs) can be differentiated into insulin-producing cells by a five-stage procedure involving altering culture conditions and addition of nicotinamide. The amounts of insulin in these cells are lower than those found in pancreatic beta cells. Glucagon-like peptide-1 (GLP-1) induces the differentiation of beta cells from ductal progenitor cells. We examined the possibility o...

Metformin lowers diabetic blood glucose primarily by reducing hepatic gluconeogenesis and increasing peripheral glucose uptake. However, possible effects by metformin on beta-cell function are incompletely understood. We speculated that metformin might positively influence insulin secretion through impacting the beta-cell nitric oxide synthase (NOS)-NO system, a negative modulator of glucose-st...

The International Society for Stem Cell Research relaxed the famous 14-day rule on culturing human embryos in its latest research guidelines.

in order to study water deficit tolerance in thymus daenensis subsp. daenensis ecotypes, seeds of eight ecotypes were collected from different locations. seedling are produced in greenhouse, and then transferred to field as an experiment of split plot design based on rcbd with three replicates. three irrigation treatments (normal irrigation, prevention of irrigation at the start of flowering an...

Crop insurance is a risk management tool with the potential of dealing with risk more efficiently, the study uses a dichotomous contingent valuation method to elicit the willingness to pay for crop insurance among cereal farmers in the Eastern region of Ghana. The study employed descriptive statistical techniques to analyse primary data obtained from 208 sampled farmers in the region. Approxima...