The drug sensitivity of four human cell lines was compared with that of Sarcoma 180 (of mouse origin) in an agar plate assay procedure. Significant differences in cell line response were found with actidione, methotrexate, puromycin aminonucleoside, strophanthin K, mitomycin C, and streptovitacin A. They ranged from 31-fold with mitomycin C to 8,500-fold with strophanthin K. The significance of...

Background: Oral feeding problems are among the most common issues in preterm infants. Various methods and feeding support tools are used in this regard, such as cup feeding and feeding nozzle. The present study aimed to determine the preferred method between cup feeding and feeding nozzle to support oral feeding in premature neonates. Methods: This clinical trial was conducted on 70 preterm i...

The aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested usi...

The hydroxyurea, a cytotoxic drug, is the mainly available therapeutical strategy for the treatment of sickle cell disease. This study aimed to evaluate the mutagenic and genotoxic potential of the hydroxyurea through the Salmonella/Microsome assay and micronucleus test in peripheral blood of mice. The doses were evaluated at 29.25-468 μmol/plate in Salmonella/Microsome assay in presence and ab...

The agar dilution (Waksman, 1943) and the agar diffusion (Foster and Woodruff, 1943b) methods of assay of streptothricin and streptomycin are the procedures most commonly employed and have led to what Waksman calls "dilution units" (Waksman, 1943) and "diffusion units" (Schatz, Bugie, and Waksman, 1944). However, in addition, Waksman speaks of "Escherichia coli units," or "Bacillus subtilis uni...

An efficient and rapid cytotoxicity assay has been developed, particularly for radiobiological studies, utilizing 96-well microtiter plates. Several days after treatment, cell numbers per well were measured by fluorescent intensity using an automatic reader after staining with the DNA specific dye Hoechst 33258. For radiobiological applications, a microtiter plate irradiation box was designed a...