Homotaurine (3-aminopropanesulfonate), a natural product and an analogue of GABA (4-aminobutyrate), was found to be a sole source of nitrogen for Cupriavidus necator (Ralstonia eutropha) H16, whose genome sequence is known. Homotaurine nitrogen was assimilated into cell material, and the quantitative fate of the organosulfonate was sulfopropanoate, which was recovered in the growth medium. The ...

* Author for correspondence: , Professor and Head, Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Hauz Khas, New Delhi-110016, India. Tel: 91-11-26596109, Fax : 91-1126582282, Email : [email protected]. Abstract. Synthetic polymers or plastics have versatile qualities of strength, lightness, and protection and were initially developed for their...

Waste paper holds great potential as a substrate for the microbial production of bioplastic (Poly-3-hydroxybutyrate (PHB)). This study aimed to produce PHB by utilizing office using Cupriavidus necator through batch and fed-batch simultaneous saccharification fermentation (SSF) approach. For experiment, different loadings shredded (3, 5 10%) with two pretreatments H2O2 (OPH) Triton X-100 (OPTH)...

LysR-type transcription regulators (LTTRs) comprise one of the largest families transcriptional in bacteria. They are typically homo-tetrameric proteins and interact with promoter DNA ~ 50–60 bp. Earlier biochemical studies have suggested that LTTR binding to bends and, upon inducer binding, bend angle is reduced through a quaternary structure change tetrameric LTTR, leading activation transcri...

Rhodospirillum rubrum possesses a putative intracellular poly(3-hydroxybutyrate) (PHB) depolymerase system consisting of a soluble PHB depolymerase, a heat-stable activator, and a 3-hydroxybutyrate dimer hydrolase (J. M. Merrick and M. Doudoroff, J. Bacteriol. 88:60-71, 1964). In this study we reinvestigated the soluble R. rubrum PHB depolymerase (PhaZ1). It turned out that PhaZ1 is a novel typ...