The influence of a standardized pollen extract (Cernitin) on the hepatic cytochrome P-450 system was studied in rats chronically exposed to an aerosol of ammonium fluoride. The content of cytochrome P-450 and cytochrome b5 and the activities of NADPH-cytochrome c reductase, aminophenazone N-demethylase, and aniline hydroxylase were determined. After 3 months the content of cytochrome b5 in the ...

A pigment-protein complex can be extracted, in aqueous 2-percent digitonin, from Euglena grown in the light. When further fractionated by acetone and ammonium sulfate this flagellate yields a c-type cytochrome. By similar extraction of dark-grown, nonphotosynthetic Euglena, another c-type cytochrome can be isolated. The cytochrome from the light-grown Euglena- is like that of cytochrome c isola...

White, David C. (The Rockefeller Institute, New York, N. Y.) Cytochrome and catalase patterns during growth of Haemophilus parainfluenzae. J. Bacteriol. 83:851-859. 1962.-By following the cytochrome and catalase concentrations during the growth cycle and under various growth conditions in Haemophilus parainfluenzae, a rapid increase in the cytochrome oxidases and a large increase in cytochrome ...

Antimycin, a specific and highly potent inhibitor of electron transfer in the cytochrome b-c1 segment of the mitochondrial respiratory chain, does not inhibit reduction of cytochrome c1 by succinate in isolated succinate-cytochrome c reductase complex under conditions where the respiratory chain complex undergoes one oxidation-reduction turnover. If a slight molar excess of cytochrome c is adde...

An antibody preparation to purified, homogeneous TPNHcytochrome c reductase from pig liver microsomes was shown to inhibit concomitantly TPNH-cytochrome c reductase (NADPH-cytochrome c oxidoreductase, EC 1.6.2.3) and ethyhnorphine N-demethylase activities in pig liver microsomes. The antibody also inhibited TPNH-cytochrome c reductase and TPNH-cytochrome P-450 reductase activities in rat and pi...

The roles of different forms of cytochrome P-450 in activation and deactivation of toxic chemicals, synthesis and breakdown of steroid hormones, and other functions, indicate the significance of these enzymes. Monooxygenase systems have been studied in species from several phyla of aquatic organisms. However, cytochrome P-450, the dominant catalyst in xenobiotic monooxygenase activity, is best ...

Small diffusible redox proteins facilitate electron transfer in respiration and photosynthesis by alternately binding to integral membrane proteins. Specific and transient complexes need to be formed between the redox partners to ensure fast turnover. In respiration, the mobile electron carrier cytochrome c shuttles electrons from the cytochrome bc1 complex to cytochrome c oxidase. Despite exte...