We report cell-to-cell movement of mitochondria through a graft junction. Mitochondrial movement was discovered in an experiment designed to select for chloroplast transfer from Nicotiana sylvestris into Nicotiana tabacum cells. The alloplasmic N. tabacum line we used carries Nicotiana undulata cytoplasmic genomes, and its flowers are male sterile due to the foreign mitochondrial genome. Thus, ...

Human immunodefi ciency virus type-1 integrase (IN) is a new and novel target for inhibitors. Strand transfer inhibitors effectively prevent concerted integration of viral DNA by IN into the host chromosomes. Raltegravir is the fi rst approved strand transfer inhibitor for the treatment of HIV-1/AIDS. We propose a mechanistic hypothesis as to "when and where" these inhibitors are active in viru...

Membrane-derived oligosaccharides (MDO) are found as soluble components of the periplasmic compartment of Escherichia coli (15). They are a family of closely related oligosaccharides containing 6 to 10 glucose units joined by 3-1,2 and 3-1,6 linkages and are variously substituted with succinic acid 0 ester, phosphoethanolamine, and sn-1-phosphoglycerol (7, 14). The mdoB locus (4, 5) encodes pho...

the possibility of occurrence of some rearrangements inside and or around coxii and atp6 genes and their relationship with male sterility in rice lines having wild abortive (wa)  cytoplasm was studied. two sterile (ir58025a and ir62829a) and two maintainer lines (ir58025b and ir62829b) were used. radioactive southern blotting was employed to carry out the experiments. the hybridization of coxii...

methods s. cerevisiae was cultured and then disrupted by sonication. after centrifugation, the harvested supernatant was considered to be a cytoplasmic extract. the protein concentration was determined by the biuret method and the extract was diluted to concentrations of 500, 1000, and 2000 µg protein/ml. the frequencies of apoptosis and necrosis were assessed in extract-treated k562 cells by e...

background: wolbachia  are common intracellular bacteria that infect different groups of arthropods including mos­quitoes. these bacteria modify host biology and may induce feminization, parthenogenesis, male killing and cyto­plasmic incompatibility (ci). recently wolbachia  is being nominated as a bio-agent and paratransgenic candidate to control mosquito borne diseases. methods: here we repor...

The insolubility of Fe3+ necessitates special mechanisms for iron acquisition in most organisms. Bacteria use siderophores to chelate Fe3+ and iron in heme, hemoglobin, transferrin and lactoferrin, and employ novel mechanisms for receptor-dependent iron transport and iron-regulated gene expression. These mechanisms involve transfer of energy from the cytoplasmic membrane to the outer membrane t...

Resistance to mercuric ions in bacteria is conferred by mercuric reductase, which reduces Hg(II) to Hg(0) in the cytoplasmic compartment. Specific mercuric ion transport systems exist to take up Hg(II) salts and deliver them to the active site of the reductase. This short review discusses the role of transport proteins in resistance and the mechanism of transfer of Hg(II) between the mercury-re...

We have used a bovine papillomavirus-based vector to generate transgenic mice. Transgenic mice result from either pronuclear or cytoplasmic injections of the vector into fertilized eggs. Of 30 mice generated by microinjection, 27 (90%) contained the vector in its episomal form, at less than one copy per cell. This represents a highly efficient means of gene transfer in which the transgene is in...