We report an automated microfluidic platform for ‘digitally’ screening the composition space of droplets containing cocktails of small molecules and demonstrate the features of this system by studying epistatic interactions between antibiotics and Escherichia coli ATCC 25922. This system has several key characteristics: (i) it uses small (<100 mL) samples of liquids and suspensions of bacteria ...

When a nitrobenzene (NB) phase came in quiescent contact with a water phase, water-droplets were formed spontaneously near the oil|water interfaces (Electrochem. Commn. 11 (2009) 239). We reported here quantitative data of the NB-droplets by use of UV-absorbance, dynamic light scattering (DLS) and voltammetry with a help of ferrocene. The supernatant separated spontaneously from the oil-water m...

BACKGROUND Autophagy is a fundamental cell biological process whereby eukaryotic cells form membranes in the cytoplasm to sequester diverse intracellular targets. Although significant progress has been made in understanding the origins of autophagosomal organelles, the source of lipids that support autophagic membrane formation remain an important open question. RESULTS Here we show that lipi...

The existence and limits of metastable droplets have been calculated using finite-system renormalization-group theory, for q-state Potts models in spatial dimension d=3. dependence the droplet critical sizes on magnetic field, temperature, number states q has calculated. same method also used calculation hysteresis loops across first-order phase transitions these systems. loop shapes deduced as...

The emerging field of droplet microfluidics requires effective on-chip handling and sorting of droplets. In this work, we demonstrate a microfluidic device that is capable of sorting picoliter water-in-oil droplets into multiple outputs using standing surface acoustic waves (SSAW). This device integrates a single-layer microfluidic channel with interdigital transducers (IDTs) to achieve on-chip...