Enzymes are the catalysts of all reactions in living systems. These reactions are catalysed in the active sites of globular proteins. The proteins are composed by amino acids with a variety of side chains ranging from non-polar aliphatic and aromatic to acidic, basic and neutral polar. This fact allows to a globular 3D protein to create in the active site all ranges of microenvironments for cat...

A modified degumming technique, involving boiling in 0.25% Na2CO3 with addition of 1% sodium dodecyl sulphate and intermittent ultrasonic agitation, was developed for knitted silk scaffolds. Sericin was efficiently removed, while mechanical and structural properties of native silk fibroin were preserved. Biocompatible and mechanically robust hybrid nano-microscaffolds were fabricated by coating...

Regenerated silk fibroin has been proposed as a material substrate for biomedical, optical, and electronic applications. Preparation of the silk fibroin solution requires extraction (degumming) to remove contaminants, but results in the degradation of the fibroin protein. Here, a mechanism of fibroin degradation is proposed and the molecular weight and polydispersity is characterized as a funct...

Lipases and phospholipases are interfacial enzymes that hydrolyze hydrophobic ester linkages of triacylglycerols and phospholipids, respectively. In addition to their role as esterases, these enzymes catalyze a plethora of other reactions; indeed, lipases also catalyze esterification, transesterification and interesterification reactions, and phospholipases also show acyltransferase, transacyla...

The purpose of the present study was to examine the physico-chemical characteristics of Moringa oleifera seeds and seed oil from a wild provenance of Pakistan. The Moringa seeds harvested from the forests of Kohat district of NWFP exhibited an oil yield of 34.80%. Protein, fiber, moisture and ash contents were 31.65, 7.54, 8.90 and 6.53%, respectively. The extracted M. oleifera seed oil reveale...

Polygalacturonases are enzymes involved in the degradation of pectic substances, being extensively used in food industries, textile processing, degumming of plant rough fibres, and treatment of pectic wastewaters. Polygalacturonase (PG) production by thermophilic fungus Thermoascus aurantiacus on solid-state fermentation was carried out in culture media containing sugar cane bagasse and orange ...

Vegetable oil is one of the basic food items which is consumed by almost every human being in this universe. Therefore quality of vegetable oil should be good enough so that it accounts for healthy life. In vegetable oil manufacturing there are four major steps involved which are neutralization, degumming, bleaching and deodorization. Among these steps bleaching is the very important and critic...

Keberadaan tanaman randu yang cukup melimpah serta kurangnya pemanfaatan minyak merupakan kesempatan besar untuk dikembangkan menjadi bahan dalam pembuatan biodiesel. Biodiesel dapat diperoleh dengan cara mereaksikan nabati alkohol menggunakan katalis pada suhu dan komposisi tertentu. Penggunaan heterogen, salah satunya CaO, biodiesel karena sifatnya lebih stabil, ramah lingkungan, korosi peral...