The global atmospheric carbon dioxide (CO₂) concentration is expected to increase continuously over the next century. However, little is known about the responses of soil bacterial communities to elevated CO₂ in terrestrial ecosystems. This study aimed to partition the relative influences of CO₂, nitrogen (N), and the spatial factor (different sampling plots) on soil bacterial communities at th...

The distribution of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in cattle, swine, and chicken manure compost was analyzed. PCR-denaturing gradient gel electrophoresis (DGGE) showed that a Candidatus Nitrososphaera gargensis-like sequence dominated in cattle manure compost, while few AOA were detected in other composts. In the case of AOB, Nitrosomonas-like sequences were detected with hi...

In the present study, a comprehensive, rapid and sensitive method for screening sequence variation of the human mitochondrial tRNA genes has been developed. For this purpose, the denaturing gradient gel electrophoresis (DGGE) technique has been appropriately modified for simultaneous mutation analysis of a large number of samples and adapted so as to circumvent the problems caused by the anomal...

With the current rapid pace at which human disease genes are identified there is a need for practical, cost-efficient genetic screening tests. Two-dimensional electrophoretic separation of PCR-amplified gene fragments on the basis of size and base pair sequence, in non-denaturing and denaturing gradient polyacrylamide gels respectively, provides a rapid parallel approach to gene mutational scan...

Marine macroalgae are subjected to large numbers of bacteria in their environment. These bacteria have the potential to affect the health and ecology of algae in a variety of ways and can be both beneficial and harmful to the algae. Therefore, algae have likely evolved mechanisms to differentially regulate the growth of bacterial species. In this study, we examined the effects of the green alga...

Abstract The hydrogen content of the two sludge samples namely TDS and PLS were increased after heat shock pretreatment from 239.23 ± 10.23 mL/L to 663.87 15.99 at 80°C 173.61 17.21 496.74 30.21 60°C, respectively. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) indicated that Enterococcus Clostridium dominant bacteria in consortium. Two strains successfully isolate...

To gain better knowledge of the effects residual chlorothalonil on soil characteristics and microbial communities, we evaluated dissipation different concentrations respiration, enzyme activities, community structure in yellow–brown loam soils. Bacterial fungal communities were examined using traditional plate counting polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE)...

Low-density lipoprotein (LDL) particles can be separated into subfractions according to size by non-denaturing polyacrylamide gradient gel electrophoresis. Established research methods require specialised equipment and are frequently unsuited to the clinical laboratory. In this study, we utilised a colour flat bed scanner in conjunction with shareware image analysis software to compare LDL part...

This work investigated biostimulation and bioaugmentation as strategies for removing polyurethane (PU) waste in soil. Soil microcosms were biostimulated with the PU dispersion agent "Impranil" and/or yeast extract or were bioaugmented with PU-degrading fungi, and the degradation of subsequently buried PU was determined. Fungal communities in the soil and colonizing buried PU were enumerated on ...