Exonuclease activity has been measured in preparations of both enzymes. The activity associated with DNA polymerase a requires denatured DNA, Mg2+ and is ATP-dependent. The polymerase b-associated nuclease requires denatured DNA and Mg2+ or Caz+. Studies of the distribution of these enzymes during the cell cycle have shown that extracts of cells harvested at the time of nuclear DNA synthesis (C...

With the introduction of the ammonium sulphate precipitation (Farr) technique, a sensitive procedure for the detection of antibodies to either native deoxyribonucleic acid (nDNA) or heat-denatured singlestranded deoxyribonucleic acid (sDNA) has become available (Wold, Young, Tan, and Farr, 1968). Furthermore, quantitation of the amount of antibody present in the test serum can be expressed with...

A study has been made of the deoxyribonucleic acid primer requirements of partially purified deoxyribonucleic acid deoxynucleotidyltransferase fractionated from regenerating liver of the adult rat. Native, double stranded DNAs derived from either viral, bacterial, or mammalian sources serve more effectively as primers than either single stranded or heat-denatured DNAs for the incorporation of d...

Triostin antibiotics, which contain a cyclic peptide with a disulphide bridge, have been prepared by growing Streptomyces triostinicus in the presence of inorganic [3PS]sulphate. The labelled triostin A has been shown to behave in all respects similarly to the authentic natural product and to enable a much more sensitive radiochemical adaptation of the solvent-partition method for determining a...

Forensic DNA (deoxyribonucleic acid) analysis or DNA profiling has played a major role in the criminal justice system. New techniques and technologies for DNA profiling continue to evolve every year. This paper reviews the literature reported during January 2011 through June 2013 in the field of forensic DNA analysis. Recent advances in almost all aspects of DNA analysis – which include sample ...

It has been asserted that recent mtDNA phylogenies support the plausibility of sympatric speciation, long considered a controversial mechanism of the origin of species. If such inferences are reliable, mtDNA phylogenies should be congruent with phylogenies based on other data. In previous work, a mtDNA phylogeny suggested that diversification of the Hawaiian cricket genus Laupala was initiated ...

The molecular weight of native vaccinia deoxyribonucleic acid (DNA) is 1 to 1.17 times that of native T4 DNA. Sedimentation of denatured vaccinia DNA through alkaline sucrose gradients yields an apparent molecular weight greater than twice that of denatured T4 DNA, implying that the complementary strands of vaccinia DNA do not separate upon denaturation. When alkali-denatured vaccinia DNA is ne...

The antibiotic bleomycin stimulates deoxyribonucleic acid (DNA) synthesis in toluene-treated Escherichia coli cells. The increase in synthesis is linear with bleomycin concentration. Bleomycin-stimulated DNA synthesis is independent of replication and dependent on DNA polymerase I. Replication is spared as the DNA polymerase I-dependent DNA synthesis increases. Bleomycin does not appear to have...

Levine, James S. (Yale University, New Haven, Conn.), and Norman Strauss. Lag period characterizing the entry of transforming deoxyribonucleic acid into Bacillus subtilis. J. Bacteriol. 89:281-287. 1965.-The kinetics of appearance of transformants as a function of time of exposure to deoxyribonucleic acid (DNA) has been studied in Bacillus subtilis. A short lag period of approximately 1 min at ...

Deoxyribonucleic acid (DNA)-DNA hybridization on nitrocellulose filters can be used to assay for replication origin DNA from Escherichia coli if the DNA attached to the filters is enriched for the replication origin sequences. Such DNA can be readily isolated from very rapidly growing cells. When low amounts of this DNA were attached to filters, radioactively labeled DNA from the replication or...