human immunodeficiency virus (hiv) transactivator tat is a potent activator of both viral and cellu lar genes. tat has also been implicated in the development of aids-related malignancy. here, we show that tat physically and functionally is able to sequester the cell cycle check point protein p53. this sequestration results in non-functional promoter activity of cyclin-dependent kinase/cyclin i...

The DNA-dependent protein kinase (DNA–PK) is a nuclear serine/threonine protein kinase that is activated upon association with DNA. Biochemical and genetic data have revealed DNA–PK to be composed of a large catalytic subunit, termed DNA–PKcs, and a regulatory factor termed Ku. In recent years, mammalian DNA–PK has been shown to be a crucial component of both the DNA double-strand break (DSB) r...

A patent inability to properly use glycolytic and fatty acid substrates and progressive insulin resistance characterize non–insulin dependent diabetes mellitus. Curiously, the failing myocardium may also share these unfavorable characteristics. Insights from the clinical arena indicate a significant relationship between insulin resistance and New York Heart Association heart failure classificat...

Here we report the cloning and functional characterization of the cyclin D-dependent kinase 4 and 6 (Cdk4/6) inhibitory protein Cdkn2d/p19Ink4d of Xenopuslaevis (Xl-Ink4d). Xl-Ink4d is the only Ink4 family gene highly expressed during Xenopus development and its transcripts were detected maternally and during neurulation. The Xl-Ink4d protein has 63% identity to mouse and human Cdkn2d/p19Ink4d ...

Abstract Glucocorticoids, such as dexamethasone and prednisolone, are widely used in cancer treatment. Different hematological malignancies respond differently to this treatment which, could be expected, correlates with outcome. In study, we have a glucocorticoid-induced gene signature develop deep learning model that can predict sensitivity. By combining expression data from cell lines patient...

Abstract Despite recent interests in developing lysine‐targeting covalent inhibitors, no general approach is available to create such compounds. We report herein a develop cell‐active inhibitors of protein kinases by targeting the conserved catalytic lysine residue using key SuFEx and salicylaldehyde‐based imine chemistries. validated strategy successfully (irreversible reversible) against BCR‐...