Gamma-tubulin is the major protein involved in the nucleation of microtubules from centrosomes in eukaryotic cells. It is present in both cytoplasm and centrosome. However, before centrosome maturation prior to mitosis, gamma-tubulin concentration increases dramatically in the centrosome, the mechanism of which is not known. Earlier it was reported that cytoplasmic gamma-tubulin complex isolate...

Background Diabetes mellitus is a global health problem representing the fifth leading cause of mortality and a major risk factor for cardiovascular diseases. In the last years, we reported an association among urinary trypsin inhibitor (UTI), a small proteoglycan that plays pleiotropic roles in many inflammatory processes, and both type 1 and 2 diabetes and developed a method for its direct qu...

Ubiquitination is a reversible modification that influences a broad range of physiological processes. There are approximately 90 DUBs encoded in the human genome, of which 79 are predicted to have catalytic activity. We have tagged 66 DUBs with GFP and systematically surveyed their sub-cellular distribution, identifying enzymes specific to the nucleus, plasma membrane, secretory and endocytic p...

The spindle assembly checkpoint monitors microtubule attachment to kinetochores and tension across sister kinetochores to ensure accurate division of chromosomes between daughter cells. Cytoplasmic dynein functions in the checkpoint, apparently by moving critical checkpoint components off kinetochores. The dynein subunit required for this function is unknown. Here we show that human cells deple...

Alkaline polyphosphate has been demonstrated to be able to reduce significant wear and friction of sliding interfaces under heavy loads (>1 GPa) and elevated temperature (800 °C and above) conditions, e.g. hot metal manufacturing. The chemical composition and fine structure of polyphosphate lubricating film is not well understood as well as the role of alkaline elements within the reaction film...

Flowers are prized as objects of great beauty and diversity, and are commercially valuable (~US$4.5 billion in international trade yearly) and highly perishable. Biologically, flower petals have an important role in the lifecycle of plants, as they protect immature reproductive structures, then provide the attraction and accessibility needed for pollination to occur. Flower petal cell wall cons...

Generating the extended endoplasmic reticulum (ER) network depends on microtubules, which act as tracks for motor-driven ER tubule movement, generate the force to extend ER tubules by means of attachment to growing microtubule plus-ends and provide static attachment points. We have analysed ER dynamics in living VERO cells and find that most ER tubule extension is driven by microtubule motors. ...

Fruit ripening is one of the developmental processes accompanying seed development. The tomato is a well-known model for studying fruit ripening and development, and the disassembly of primary cell walls and the middle lamella, such as through pectin de-methylesterified by pectin methylesterase (PE) and depolymerization by polygalacturonase (PG), is generally accepted to be one of the major cha...

Attempts to understand and control the formation of levoglucosan, as a key bio-oil compound an important intermediate chemical, during biomass pyrolysis have recently generated large number experimental computational studies. Whilst promising mechanisms been put forward explain levoglucosan formation, there has yet be clarity on factors that inhibit stoichiometric yields from cellulose, and, cu...