Plasma and cell-derived fibronectin are potent chemoattractants for human dermal fibroblasts in vitro. The chemotactic property of fibronectin resides in a major 140,000-mol wt non-gelatin-binding fragment of the native molecule. Human monocytes and neutrophils do not recognize fibronectin as a chemotactic stimulus. These findings suggest that fibronectin and perhaps certain fragments of fibron...

The molecular circadian clock, which consists of transcription-translation feedback loops, is robust in all skin layers. As dysfunction associated with diseases, understanding the clock may provide insights into disease development and management. Particularly, core machinery interacts elements wound healing process such as fibroblast migration, manipulation network or alternation wounding time...

Tissue engineering is a multidisciplinary field an interdisciplinary for the design of biological substitutes that can improve, restore, and maintain tissue functions. Thermoplastic polyurethanes (TPUs) are linear polymers which widely used due to its flexibility in processing methods, biocompatibility excellent mechanical properties. They suitable materials use three-dimensional (3D) printing....

A skin equivalent model has been used to fabricate tissues with psoriatic and normal cells. Psoriatic fibroblasts can induce hyperproliferative activity in normal keratinocytes. The psoriatic epidermis from lesions continues to proliferate at high rates for at least 15 days in this model, and normal fibroblasts are unable to suppress this hyperproliferation. The primary defect in psoriatic skin...

Objective — to assess the efficacy and safety of intradermal injections allogeneic fibroblasts into non-healing wounds in a patient with junctional epidermolysis bullosa. Material Methods A 49-year-old intermediate bullosa was injected intradermally base 1 mL suspension fibroblasts, which contained 5×106 cells/mL, 10×106 20×106 cells/mL. Immunofluorescence mapping exhibited reduced β3 chain lam...

OBJECTIVE To determine the effects of increasing concentrations of methotrexate on the proliferation and glycosaminoglycan (GAG) synthesis of cultured dermal fibroblasts from patients with scleroderma. METHODS Cultured dermal fibroblasts from nine patients with scleroderma and nine normal volunteers were grown for 72 hours in media containing various concentrations of methotrexate. The GAG sy...

Cultured human dermal fibroblasts treated with immune interferon express HLA-DR antigens. We report here that DRpositive fibroblasts present tetanus toxoid (TT) to autologous TT-specific monoclonal helper T cells vigorously depleted of monocytes by passage over Sephadex G10 columns followed by treatment with the monoclonal antibodies (mAb) OKMl and Leu Ml plus complement. The extent of T cell p...

OBJECTIVE Glycogen synthase kinase 3β (GSK-3) regulates the phosphorylation and subsequent degradation of β-catenin, thereby preventing aberrant activation of the canonical Wnt pathway. A study was undertaken to define the role of GSK-3 in fibroblast activation and in experimental models of systemic sclerosis (SSc). METHODS siRNA and specific inhibitors were used to inhibit GSK-3 in cultured ...