Whole-genome amplification (WGA) techniques are used for non-specific amplification of low-copy number DNA, and especially for single-cell genome and transcriptome amplification. There are a number of WGA methods that have been developed over the years. One example is degenerate oligonucleotide-primed PCR (DOP-PCR), which is a very simple, fast and inexpensive WGA technique. Although DOP-PCR ha...

Opioid agonists have a broad range of effects on cells of the immune system, including modulation of the inflammatory response, and opioid and chemokine receptors are co-expressed by many white cells. Hetero-oligomerization of the human DOP opioid and chemokine CXCR2 receptors could be detected following their co-expression by each of co-immunoprecipitation, three different resonance energy tra...

We aimed at elucidating the influence of shear-induced structures (shear-enhanced concentration fluctuations and/or shear-induced phase separation), as observed by rheo-optical methods with small-angle light scattering under shear flow (shear-SALS) and shear-microscopy, on viscoelastic properties in semidilute polystyrene (PS) solutions of 6.0 wt % concentration using dioctyl phthalate (DOP) as...

Weather and climate parameters and their effects on crops, is one of the most effective factors in yield increase. Agro-climatic studies could be used to determine the potential of various regions and the exploit these resources to the maximum possible extent. In this study, in order to evaluate the agricultural climate of spring canola cultivation in selected stations in Hamedan province, the ...

LFG-DOP (Bod and Kaplan, 1998, 2003) provides an appealing answer to the question of how probabilistic methods can be incorporated into linguistic theory. However, despite its attractions, the standard model of LFG-DOP suffers from serious problems of overgeneration because (a) it is unable to define fragments of the right level of generality, and (b) it has no way of capturing the effect of an...

A novel, simple, sensitive and effective method has been developed for preconcentration of Hg(II) on nano-Fe3O4-DOP-ED solid-phase extraction adsorbent. In this paper, Dioctyl phthalate (DOP) was used to encapsulate nano-Fe3O4 and produce a nano-Fe3O4-DOP sorbent based new sorbent was prepared. This was treated with ethylenediamine (ED) in another solvent-free procedure for the formation of a n...

Post-translational modification of proteins with prokaryotic ubiquitin-like protein (Pup) is the bacterial equivalent of ubiquitination in eukaryotes. Mycobacterial pupylation is a two-step process in which the carboxy-terminal glutamine of Pup is first deamidated by Dop (deamidase of Pup) before ligation of the generated γ-carboxylate to substrate lysines by the Pup ligase PafA. In this study,...

We propose a generalization of the supervised DOP model to unsupervised learning. This new model, which we call U-DOP, initially assigns all possible unlabeled binary trees to a set of sentences and next uses all subtrees from (a large subset of) these binary trees to compute the most probable parse trees. We show how U-DOP can be implemented by a PCFG-reduction technique and report competitive...

| This paper describes the use of a semi-continuous hidden Markov models for speaker veriication. The system uses a technique for discriminative hidden Markov modelling known as discriminating observation probabilities (DOP). Results are presented for text-dependent experiments on isolated digits from 25 genuine speakers and 84 casual im-poster speakers, recorded over the public telephone netwo...

In Caenorhabditis elegans, the dopamine transporter DAT-1 regulates synaptic dopamine (DA) signaling by controlling extracellular DA levels. In dat-1(ok157) animals, DA is not taken back up presynaptically but instead reaches extrasynpatic sites, where it activates the dopamine receptor DOP-3 on choligeneric motor neurons and causes animals to become paralyzed in water. This phenotype is called...