The thermal reaction of olefins with nitrous oxide was recently put forward as a promising synthetic ketone source. The 1,3-dipolar cycloaddition of N(2)O to the C=C double bond, forming a 4,5-dihydro-[1,2,3]oxadiazole intermediate, was predicted to be the first elementary reaction step. This oxadiazole can subsequently decompose to the desired carbonyl product and N(2)via a hydrogen shift. In ...

Oxidation-responsive oligonucleotides (ONs) can exhibit controlled functions by sensing dysregulated oxidation conditions in living systems. Herein, we describe a postmodification approach to constructing an oxidation-responsive ON that takes advantage of the reactivity diazo compounds introducing 4-boronobenzyl group phosphate at 5′-terminal ON. We

Purpose: To develop and validate a simple visible spectrophotometric method for the quantitative determination of ganciclovir in bulk sample and dosage form. Method: The method was based on the diazo coupling reaction between diazotized ganciclovir and acidified p-dimethylaminobenzaldehyde. Various analytical parameters for the azo adduct were established. Validation of the new method was carri...

ROPER CLINICAL MANAGEMENT of infants with erythroblastosis fetalis necessitates the determination of total serum bilirubin. Of the many modifications of the original diazo reaction on blood serum (1) a micromethod using capillary blood is preferable. One tenth miffiliter of serum was used by Davidson et al. (2) in their modification of the Ernst and F#{246}rster (3) method and by With (4, 5) in...

Guidelines for managing hyperbilirubinemia in newborns were developed by using diazo methods that measure total and direct-reacting bilirubins and calculate an indirect fraction. The automated Kodak Ektachem system allows for measuring serum bilirubin by either of two dry-slide methods: TBIL, involving a modified diazo method, and NBIL, involving a dual-wavelength colorimetric method that fract...