A search was undertaken for bacteria which degrade chondroitin sulfate in nature and to find bacteria with a usefully high rate of chondroitinase (ChSase) productivity. First, 253 ChSase-producing bacteria were obtained from aquatic and land environments in Japan by aerobic and anaerobic screening methods. Identification according to Bergey's Manual of Determinative Bacteriology or Bain and She...

The 2-fluoro derivative of 9-beta-D-arabinofuranosyladenine (2-F-ara-A) and its soluble 5'-formate and 5'-phosphate derivatives were therapeutically effective against the parent leukemia L1210 (L1210/0). 2-F-ara-A and 9-beta-D-arabinofuranosyladenine 5'-formate were inactive aginst a 1-beta-D-arabinofuranosylcytosine-resistant subline (L1210/ara-C) that was deficient in deoxycytidine kinase. De...

Parke-Davis bovine thrombin was resolved into three distinct protein peaks on a Sephadex G-200 column. A stepwise chromatographic technique on diethylaminoethyl (DEAE) cellulose was developed for the complete separation and partial purification of thrombin and activated Factor X. Thrombin was quantitatively eluted in the first protein peak with 0.1 M NaCl, pH 7.0. A second peak with residual th...

Parke-Davis bovine thrombin was resolved into three distinct protein peaks on a Sephadex G-200 column. A stepwise chromatographic technique on diethylaminoethyl (DEAE) cellulose was developed for the complete separation and partial purification of thrombin and activated Factor X. Thrombin was quantitatively eluted in the first protein peak with 0.1 M NaCl, pH 7.0. A second peak with residual th...

Three proteinases which digest hemoglobin rapidly at acid pH (3.5 to 4.5) were identified in crude extracts of soybean (Merr.) leaves and separated by chromatography on DEAE-cellulose. All three enzymes were endopeptidases as judged by the ratio of alpha-amino-nitrogen plus peptide nitrogen over alpha-amino-nitrogen in the trichloroacetic acid-soluble portion of hemoglobin digests. Proteinase I...

Threonyl ribonucleic acid synthetase was purified 400-fold from rat liver. During stepwise chromatography on diethylaminoethyl cellulose, two fractions with this enzymatic activity were observed. The separated fractions give distinct elution patterns on rechromatography on the same ion exchanger. Threonyladenylate-enzyme complex prepared with 14Cthreonine and 3H-adenosine triphosphate shows a s...

Two distinct aromatase-active protein complexes are solubilized by use of deoxycholate and separated by diethylamino-ethyl-cellulose chromatography from lyophilized powder of 900 X g precipitate fraction of human term placenta. Aromatase activity to produce estriol, the major estrogen of human pregnancy, was designated to be aromatase I activity and measured by estriol formation from 16 alpha-h...

Serum and serum fractions from patients with rheumatoid arthritis have been subjected to anion exchange chromatography on diethylaminoethyl cellulose. The rheumatoid factor activity was clearly separated from the bulk of the gamma globulin by virtue of the anomalous behavior of macroglobulins on this column. Purification of rheumatoid factor by precipitation with fraction II, resolution in 4 M ...

HE detection, by agar gel electrophoresis, of as many as ten alcohol dehydrogenase isozymes in Drosophila melanogaster (URSPRUNG and LEONE 1965) presents a problem in the interpretation of genetic and epigenetic control of synthesis and function of multiple forms of enzymes. The observation that the three slowest cathodalIy migrating isozymes show a more intensive formazan staining when primary...

Guinea pig liver transglutaminase has been purified 230fold in high yield by means of diethylaminoethyl cellulose chromatography of liver homogenate supernatant fluid, precipitation of the enzyme with protamine, selective extraction with ammonium sulfate solution, and rechromatography on the cellulose absorbent. Estimates of molecular weight made by several procedures, 76,900 f 5,000 (sedimenta...