as analyte concentrations increased, within-and between-day imprecision decreased. The CVs of these samples, when assay dilution was accounted for, were consistent with the low (ng/L) LOQ estimated for each SMIA. Highly sensitive and precise SMIAs with excellent recoveries for several other analytes were also developed (Table 1 and see Fig. S1C in the online Data Supplement). All LODs were Ͻ1 n...

Differential Scanning Calorimetry (DSC) has been used in the past to study the thermal unfolding of many different viruses. Here we present the first DSC analysis of rabies virus. We show that non-inactivated, purified rabies virus unfolds cooperatively in two events centered at approximately 62 and 73 °C. Beta-propiolactone (BPL) treatment does not alter significantly viral unfolding behavior,...

The thermal denaturation of aspartate transcarbamoylas of Escherichia coli was investigated by differential scanning calorimetry. Isolated regulatory and catalytic subunits were heat denatured at 55 and 80 degrees C, respectively. In contrast, the intact enzyme was denatured in two steps. A small endotherm near 73 degrees C was assoicated with denaturation of the regulatory subunits and the maj...

Phaseolus vulgaris phytohemagglutinin L is a homotetrameric-leucoagglutinating seed lectin. Its three-dimensional structure shows similarity with other members of the legume lectin family. The tetrameric form of this lectin is pH dependent. Gel filtration results showed that the protein exists in its dimeric state at pH 2.5 and as a tetramer at pH 7.2. Contrary to earlier reports on legume lect...

The thermoluminescence (TL) glow curves of UV irradiated PAAm-PVA polyblend have been studied. The experimental glow curves show the presence of two peaks with glow peak temperatures at 373K and 479K. The values of activation energy and frequency factors are determined by employing computer simulation. Most of the TL glow curves obey non first order kinetics. The results obtained by using TL ex...