Single-cell analysis has become the interest of a wide range of biological and biomedical engineering research. It could provide precise information on individual cells, leading to important knowledge regarding human diseases. To perform single-cell analysis, it is crucial to isolate the individual cells before further manipulation is carried out. Recently, microfluidic biochips have been widel...

Label-free sensing is a promising approach for point-of-care testing devices. Among optical transducers, photonic crystal slabs (PCSs) have positioned themselves as an inexpensive yet versatile platform label-free biosensing. A spectral resonance shift observed upon biomolecular binding to the functionalized surface. Commonly, PCS read out by spectrometer. Alternatively, may be translated into ...

Quantification of mRNA in single cells provides direct insight into how intercellular heterogeneity plays a role in disease progression and outcomes. Quantitative polymerase chain reaction (qPCR), the current gold standard for evaluating gene expression, is insufficient for providing absolute measurement of single-cell mRNA transcript abundance. Challenges include difficulties in handling small...

Novel latching microfluidic valve structures are developed, characterized, and controlled independently using an on-chip pneumatic demultiplexer. These structures are based on pneumatic monolithic membrane valves and depend upon their normally-closed nature. Latching valves consisting of both three- and four-valve circuits are demonstrated. Vacuum or pressure pulses as short as 120 ms are adequ...

In this paper we describe a newly developed maskless projection system for lithography based on a digital mirror device and its use for the rapid creation of microfluidic structures in a polytetrafluoroethylene (PTFE) like highly fluorinated monomer.

This paper presents a microsystem for biomolecular recognition (DNA, enzymes) based on a biochip, which uses magnetic field arraying of magnetically tagged biomolecules and high sensitivity sensors for single biomolecule detection. The microsystem provides the electronic circuitry for addressing, reading out and sensing each of the 16× 16 magnetoresistive sensors that compose the biochip. A pro...

A universal design for a biochip that reads out DNA en coded graphs is enhanced by a readout technique that may resolve mul tiple solutions of Hamiltonian path problems A single laboratory step is used DNA encoded graphs are labeled with many quantum dot barcodes and then hybridized to the universal biochip Optical readouts one for each barcode yield multiple partial readouts that may isolate i...

This paper reports on the use of a digital microfluidic platform to perform multiplex automated genetic engineering (MAGE) cycles on droplets containing Escherichia coli cells. Bioactivated magnetic beads were employed for cell binding, washing, and media exchange in the preparation of electrocompetent cells in the electrowetting-on-dieletric (EWoD) platform. On-cartridge electroporation was us...