Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a λ=2.48 nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, ...

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Reconfiguration is a computational algorithm of adaptively updating computer generated holograms (CGHs) for the positional change of an observer's viewing window with low computational load by efficiently using pre-calculated elementary CGHs. A fast reconfiguration algorithm of CGHs for three-dimensional mesh objects is proposed. Remarkable improvement is achieved in the computation speed of CG...

this study was conducted to determine the use of three-dimensional ultrasonography (3du) in ocular imaging of the dog and measuring of its optical axis. 12 healthy mixed-breed dogs including 6 males and 6 females were studied. 3du of the eyes were done using a 5–12 mhz linear trapezoid transducer. 3du of the eyes were evaluated and the normal optical long axis through a line between the cornea ...

Microscopy has incited many of the greatest revolutions in scientific history. In particular, these inventions have sparked paradigm shifts in biology, and discoveries like the structure of DNA are indebted to such studies. The development of the field spans from recent work like scanning electron microscopy and atomic force microscopy to the original optical microscope, which dates back hundre...

This paper presents a model of a virtual laboratory for an introductory computer science course. The proposed model aims at solving a number of problems involved in the educational procedure of such a course. The model architecture consists of seven modules, each one corresponds to a specific topic of the course. Every module provides several different services in order to assist students to as...

We demonstrate the direct three-dimensional imaging of densely packed colloidal nanostructures using stimulated emission depletion microscopy. A combination of two de-excitation patterns yields a resolution of 43 nm in the lateral and 125 nm in the axial direction and an effective focal volume that is by 126-fold smaller than that of a corresponding confocal microscope. The mapping of a model s...

This paper presents a comparison between Radio Tomographic Imaging (RTI) and Device-free Passive (DfP) tracking in standard WiFi networks. We show that for typical WiFi deployments, tracking methods are able to extract location information from a limited number of streams while imaging methods fail to produce meaningful images. On the other hand, we show that when the number of streams increase...

OBJECTIVE The use of stone and plaster study models is an integral part of any dental practice and is required for research. Storage of study models is problematic in terms of space and cost. Ayoub et al.(1) introduced a new technique based on the recent advances in stereophotogrammetry for archiving dental study models in a digital format. However, assessment of the accuracy of the generated t...

In this study, we present confocal Raman microscopy for chemically selective analysis of a human buccal epithelial cell layer with a focus on label-free visualization of particle uptake into the cells. We demonstrate the suitability and benefit of this analytical technique in comparison to confocal fluorescence microscopy for three dimensional imaging of in vitro cell models.