The direct agglutination test (DAT) based on a freeze-dried antigen and the rK39 dipstick test were evaluated for the sero-diagnosis of visceral leishmaniasis (VL). The sensitivity and specificity of both tests were determined using sera from confirmed VL patients (n = 21), healthy controls (n = 19) and from patients with other confirmed infectious diseases (n = 42). The DAT had a sensitivity a...

A comparison is made of tests for thyroglobulin antibody, using gel diffusion, electroprecipitin, bentonite flocculation, tanned red cell agglutination, and latex slide agglutination techniques on sera from cases of Hashimoto's disease and other thyroid disorders. Any increase in gamma globulin was also noted from the serum electrophoretic pattern. The gel diffusion and electroprecipitin tests ...

background: the aim of present study was to determine the seroprevalence of canine visceral leishmaniasis (cvl) among stray and owned dogs in kouhsar district of alborz province, central iran. methods: the study was performed from march 2011 to july 2011 using direct agglutination test (dat). three hundred and thirty seven dogs including 257 stary and 80 owned dogs were selected by random sampl...

background: visceral leishmaniasis (vl) or kala azar is an infectious disease caused by various species of leishmania parasites. the aim of this study was to detect and compare the presence of anti-leishmania antibodies in the urine of vis-ceral leishmaniasis patients using elisa and dat methods. methods: a total of 30 urine samples were collected from vl patients referred to shiraz (southeast ...

The performance of a direct sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was compared with that of the Pastorex Aspergillus antigen latex agglutination (LA) test by using 532 serum samples from 61 patients at risk for invasive aspergillosis. The ELISA gave positive results earlier in the course of infection than did the LA test. A sensitivity of 70%...

Blood group A + H substance purified from hog gastric mucin was fixed to polystyrene particles by physical absorption to produce a lectin-agglutinable latex. Agglutination of this latex by soybean agglutinin, concanavalin A, and wheat germ agglutinin is specifically inhibited by the respective monosaccharide haptens and is not affected by known noninhibitory sugars. The sensitivity of the assay...

Recent research into the nature and significance of rheumatoid serum factors has been largely immunological and biochemical. The epidemiology of these components is virtually unknown. During recent years we have had the opportunity of studying several population samples, and data from these surveys have now been gathered and are presented with particular reference to the epidemiology of the ser...

The diagnosis of visceral leishmaniasis involves isolation of the parasites in culture or invasive and risky techniques including demonstration of parasite in stained preparations of relevant tissues such as spleen, bone marrow or lymph node by light microscopic examination remains a gold standard. Serological tests using rK39 in ELISA or rapid immunochromatographic tests, direct agglutination ...