Mammalian cells treated with ultraviolet (UV) light provide one of the best-known experimental systems for depicting the biological consequences of DNA damage. UV irradiation induces the formation of DNA photoproducts, mainly cyclobutane pyrimidine dimers (CPDs) and (6-4) pyrimidine-pyrimidone photoproducts [(6-4)PPs], that drastically impairs DNA metabolism, culminating in the induction of cel...

In order to examine the relationship between accumulation of residual DNA double-strand breaks (DSBs) and cell death, we have used a control and an ATM (Ataxia-Telangiectasia Mutated) defective cell line, as Ataxia-Telangiectasia (AT) cells tend to accumulate residual DSBs at long times after damage infliction. After irradiation, AT cells showed checkpoint impairment and a fraction of cells dis...

Oligodeoxynucleotide (ODN) sequences containing unmethylated cytidine phosphate guanosine (CpG) motifs prevalent in bacterial DNA attenuate allergic lung inflammation in experimental models of asthma but failed to inhibit eosinophilia and improve lung function in patients with asthma. Bacterial respiratory tract infections exacerbate asthma in humans. Increased eosinophil survival is a critical...

Background Freezing process has destructive effect on different functions of sperm. Therefore, sperm motility was reduced and apoptosis and DNA damage and also levels of reactive oxygen species (ROS) were increased. Insulin-like factor-3 (INSL3) is a member of Relaxin family and directly affects on spermatogenesis and also plays a protective role against apoptosis. Therefore, with due regard to...

hepatitis b virus (hbv) dna polymerase transactivated protein 1 (hbvdnaptp1) is a novel protein transactivated by hbv dna polymerase, screened by suppression subtractive hybridization technique (genbank accession no: ay450389). the biological function of hbvdnaptp1 was investigated in this study.we constructed a vector pcdna3.1 (-)/myc-his a-hbvdnaptp1 and used it to transfect acute monocytic l...

fragrant species of the genus salvia have been attributed many medicinal properties, which include anticancer activity. in the present study, cytotoxic properties of total methanol extract of salvia chloroleuca rech. f. & aellen and its fractions were investigated on mcf-7, a breast carcinoma cell line. malignant and non-malignant cells were cultured in rpmi medium and incubated with different ...

The molecular changes responsible for inducing neuronal apoptosis are unknown. Rat cortical neurons were treated with x-irradiation 7 d after isolation to test for the role of DNA damage in neuronal death. The response of neurons to x-irradiation was compared with that of astrocytes that had been isolated 3 weeks earlier from newborn rats. At the time of irradiation, the neurons appeared well d...

We previously reported that apoptosis is increased in smooth muscle cells cultured from the aorta of spontaneously hypertensive rats versus normotensive controls. As an initial in vivo exploration, we now examined smooth muscle cell apoptosis regulation during the regression of vascular hypertrophy in the thoracic aorta media of spontaneously hypertensive rats receiving the antihypertensive dru...

Pluripotent stem cells must strictly maintain genomic integrity to prevent transmission of mutations. In human induced pluripotent stem cells (iPSCs), we found that genome surveillance is achieved via two ways, namely, a hypersensitivity to apoptosis and a very low accumulation of DNA lesions. The low apoptosis threshold was mediated by constitutive p53 expression and a marked upregulation of p...

PurposeMammography is the diagnostic imaging practice used in screening to detect early lesions suspected of malignancy. It uses a low energy X-ray beam which dose order 2–3 mGy delivered patient breast cells. However, it has been speculated that could lead significant cell damage, when compared conventional X-ray. We investigated biological effects doses, with mean glandular doses (MGDs) 2.5 a...