Drosophila cells in culture can be transformed by introducing exogenous DNA carrying a selectable marker. Here we report on the fate of plasmids that contain an extended fragment of Drosophila DNA in addition to the selectable marker. A small minority of the resulting transformants appear to arise from homologous recombination at the chromosomal target. However, the majority of the insertions a...

As a great number of the genomic DNA sequences can now be generated in a day, the demand for a very fast and accurate method for positioning genomic DNA sequences on a genome is high. A unique marker is a sequence which appears only once in a genome. The unique marker method is an efficient method to perform this task. The amount of time needed for positioning genomic DNA sequences grows rapidl...

Detection of molecular markers for colorectal neoplasia in feces has the potential to improve performance of simple noninvasive screening tests for colorectal cancer. Most research has explored the value of DNA-based, RNA-based, and protein-based markers. In all cases there has been a trend to move from a single marker to a panel of markers to improve sensitivity. Unfortunately, no type of mole...

plant breeders use rye (secale cereale l.) as a foreign genetical resource tool in increasing wheat genetical variations. 1r chromosome and especially its short arm, 1rs has been introduced into wheat genome frequently. 1rs studies and effective selection is used in cytogenetical, chemicals techniques and dna markers. present study was conducted to investigate the presence of rye chromosome seg...

The ability of peptide nucleic acid (PNA) to open up duplex DNA in a highly sequence-specific manner makes it possible to detect short DNA sequences on the background of or within genomic DNA under non-denaturing conditions. To do so, chosen marker sites in double-stranded DNA are locally opened by a pair of PNA openers, thus transforming one strand within the target region (20-30 bp) into the ...

BACKGROUND The purpose of our study was to evaluate if DNA methylation level in leukocytes may be used as a surrogate marker of genome methylation status in laryngeal cancer tissues. METHODS We evaluated global DNA methylation using an ultra performance liquid chromatography (UPLC)-based method to assess the total content of 5-methylcytosine (5 mC). RESULTS The study was performed on DNA is...

In this paper, the genetic diversities of 12 rice genotypes (Oryza sativa L.), representing Indica, Japonica, and Indica–Japonica varieties, were assessed using twelve ISSR five SSR markers. addition, evaluated for 11 agro-morphological traits in a two-year trial. Association mapping was performed to detect any association between DNA markers traits. An analysis conducted considering relative k...

The thrips Scirtothrips dorsalis Hood (Thysanoptera: Thripidae) is an invasive pest that poses a significant economical threat to U.S. agriculture and trade. In this study, DNA sequence data and polymerase chain reaction (PCR) were utilized to develop a molecular diagnostic marker for S. dorsalis. The DNA sequence variation from the internal transcribed spacer 2 (ITS2) region of nuclear ribosom...

BACKGROUND DNA barcoding is a promising tool to facilitate a rapid and unambiguous identification of sponge species. Demosponges of the order Dictyoceratida are particularly challenging to identify, but are of ecological as well as biochemical importance. METHODOLOGY/PRINCIPAL FINDINGS Here we apply DNA barcoding with the standard CO1-barcoding marker on selected Indo-Pacific specimens of two...

The progress made in DNA marker technology has been tremendous and exciting. DNA markers have provided valuable tools in various analyses ranging from phylogenetic analysis to the positional cloning of genes. The development of high-density molecular maps which has been facilitated by PCR-based markers, have made the mapping and tagging of almost any trait possible. Marker-assisted selection ha...