1. Abstract 2. Introduction 3. Genotyping methods 3.1. Genetic markers 3.1.1. Insertion sequences 3.1.2. Short repetitive DNA sequences 3.2. Restriction fragment length polymorphism analysis 3.2.1. IS6110 RFLP analysis 3.2.2. PGRS RFLP analysis 3.3. PCR-based typing 3.3.1. Mixed-linker PCR 3.3.2. Spoligotyping 3.3.3. VNTR and MIRU-VNTR typing 3.4. Comparison of genotyping methods 4. Molecular e...

background: fusobacterium necrophorum as a non-spore-forming gram-negative anaerobic bacillus is an important human and animal pathogen. it may cause severe systemic infections (lemierre's syndrome) and some other infections. the aim of this study was to subtype fusobacterium necrophorum by using pcr methods. materials and methods: twenty five strains of fusobacterium necrophorum subspecies fun...

AIM A rapid analysis of mitochondrial DNA (mtDNA) sequences with an array of immobilized sequence-specific oligonucleotide (SSO) probes was tested on 18 skeletal elements recovered from mass graves in Croatia, which could not be genotyped with common forensic nuclear DNA systems (PM+DQA1 and short tandem repeat analysis). METHODS We used duplex polymerase chain reaction (PCR) amplification of...

A second-generation signal amplification, nucleic acid-based test for the rapid detection and typing of herpes simplex virus (HSV) DNA was developed and evaluated with artificial and clinical specimens. The analytical sensitivity of the Hybrid Capture II (HC II) HSV DNA assay was determined by testing either cloned HSV DNA or total genomic HSV DNA titrations and resulted in detection thresholds...