A panel of 20 human myelin basic protein (hMBP)-specific T-lymphocyte lines was generated from the peripheral blood of eight multiple sclerosis (MS) patients and two healthy donors, most of them expressing the HLA-DR2 haplotype, which is associated with an increased susceptibility to MS. Using HLA-DR gene-transfected mouse L-cell lines as antigen-presenting cells, we established that of the 20 ...

We investigated the role of the complementarity determining region 1 (CDR1) of T cell receptor (TCR) beta chain both in antigen/major histocompatibility complex I (MHC I) and in superantigen (SAg)/MHC II complex recognition. Residues 26 to 31 of the V beta 10 domain of a TCR derived from an H-2Kd-restricted cytotoxic clone were individually changed to alanine, using site-directed mutagenesis, a...

Extended radio sources are an important minority population in modern deep surveys, because they enable detailed investigation of the physics governing radio-emitting regions such as active galaxies and their environments. Cross-identification with optical host is challenging for this extended population, due to morphological complexity multiple potential counterparts. In first data release Low...

Aims. We present the first public release of high-quality data products (DR1) from Hi-GAL, the Herschel infrared Galactic Plane Survey. Hi-GAL is the keystone of a suite of continuum Galactic plane surveys from the near-IR to the radio and covers five wavebands at 70, 160, 250, 350 and 500 μm, encompassing the peak of the spectral energy distribution of cold dust for 8 <∼ T <∼ 50 K. This first ...

Two approximately 135-nucleotide (nt) direct repeats flank the Rous sarcoma virus (RSV) oncogene src and are composed of two smaller repeats, dr1 (approximately 100 nt) and dr2 (approximately 36 nt). These sequences have been reported to contain cis-acting signals necessary for RNA packaging and elements that allow cytoplasmic accumulation of unspliced RNA (cytoplasmic transport elements). In t...

BACKGROUND Major histocompatibility complex proteins are believed to undergo significant conformational changes concomitant with peptide binding, but structural characterization of these changes has remained elusive. METHODOLOGY/PRINCIPAL FINDINGS Here we use molecular dynamics simulations and experimental probes of protein conformation to investigate the peptide-free state of class II MHC pr...

We present the results from an automated search for damped Lyα (DLA) systems in the quasar spectra of Data Release 1 from the Sloan Digital Sky Survey (SDSS-DR1). At z ≈ 2.5, this homogeneous dataset exceeds the statistical significance of the previous two decades of research. We derive a statistical sample of 71 damped Lyα systems (> 50 previously unpublished) at z > 2.1 and measure H I column...

It has been suggested that self major histocompatibility complex (MHC) peptides bound to self MHC molecules may be involved in the intrathymic induction of self tolerance. We studied the antigenicity of synthetic peptides derived from the first domain of DR beta 1*0101 chain in a DR beta 1*0101 responder. We found that a peptide corresponding to residues 21-42 of the beta chain could elicit the...

1 REGIONAL GEOLOGY 2 The northern California Coast Ranges are composed the Franciscan Complex, a penetratively 3 sheared set of metasedimentary rocks forming an accretionary prism. The Franciscan complex is 4 subdivided into three broad belts that young to the west: the Eastern, Central and Coastal belts, 5 reflecting the cumulative accretion of oceanic sediments to western North America (McLau...