Plumbagin (PLB) has been shown to have anticancer activities in animal models, but the role of PLB in prostate cancer treatment is unclear. This study aimed to investigate the effects of PLB on apoptosis and autophagy and the underlying mechanisms in human prostate cancer cell lines PC-3 and DU145. Our study has shown that PLB had potent pro-apoptotic and pro-autophagic effects on PC-3 and DU14...

The antiparasitic drug, suramin, has antiproliferative effects in human carcinoma cells. It has been suggested that this occurs through blockade of growth factor-receptor interactions. Three types of evidence that suramin rapidly inhibits cellular respiration or disrupts cellular energy balance in intact cells of the human prostate carcinoma cell line, DU145, are presented. Beginning at approxi...

Neutral endopeptidase (NEP) is a cell-surface peptidase that inhibits prostate cancer cell growth partly via inhibition of Akt kinase. We investigated the antitumor effects of an adenovirus gene delivery system (AdNEP) to restore NEP expression in DU145 prostate cancer cells in combination with paclitaxel chemotherapy. DU145 cells were infected with adenovirus expressing NEP or LacZ, treated wi...

Activins are growth and differentiation factors which have been shown to have proliferative and antiproliferative actions in many tissues. In addition, they have been implicated in tumourigenesis in reproductive tissues. Although activin and inhibin are present in rat ventral prostate, inhibin beta, but not alpha, subunit proteins have been detected in the human prostate epithelial tumour cell ...

Cancers cells change their mechanical properties to migrate through crowded multicellular environments. Metastatic cells’ traction forces have been shown increase substantially relative those of non-metastatic cells, and this contractility is further increased by matrix stiffening, promoting tumor phenotypes. These findings suggest a relationship between cell contractility, microenvironment sti...

Previous studies have reported that the expression levels of microRNA (miR)?28?3p are downregulated in prostate cancer (PCa) compared with those adjacent normal tissues. However, to best our knowledge, function and underlying mechanisms miR?28?3p PCa not been reported. The present study aimed explore role its mechanism development PCa. In study, ADP?ribosylation factor 6 (ARF6) were analyzed us...

Of six prostatic carcinoma cell lines examined (ALVA31, DU145, JCA1, LNCaP, ND1, and PC3) by flow cytometric analysis, all were found to be positive for Fas antigen. Furthermore, of the prostate tissue specimens studied (six cases), all revealed Fas expression in benign and malignant epithelial cells. The agonistic anti-Fas monoclonal antibody (IPO-4) induced apoptosis in only two of six cell l...

objective(s): to investigate the efficacy of interleukin 11 (il-11) towards the high dose methotrexate (hdmtx)-concurrent rat small intestinal mucositis and its impacts on the proliferation of the human t-lymphoblastic leukemia (cem) cell line. materials and methods:95 wistar rats were randomly divided into five groups, the normal control group (a), the methotrexate (mtx) control group (b), the...

background and objective: the management of apoptotic cell death has been considered as a putative therapeutic strategy for cancer treatment. in the present study we investigated the putative pro-apoptotic effect of allicin, the main garlic organosulfur component with repeatedly claimed chemopreventive potency, on the human adenocarcinoma cell line ht29 as an apoptosis resistant cell line, in v...

the cytotoxicity of the biomaterials is a key issue that should be addressed prior to pre-clinical applications. this study was designed to evaluate and compare the cytotoxixity of two forms of bioactive glasses:nanopowder:andmicropowder. human hgf1-pi53 gingival fibroblast cells were used to evaluate the cytotoxicity of 0.5, 1, 1.5, 2, 5, 10, 15 and 20 mg/ml concentrations of the two bioactive...