Thymidylate synthase (TS) catalyzes the methylation of dUMP to dTMP and is the target for the widely used chemotherapeutic agent 5-fluorouracil. We used random sequence mutagenesis to replace 13 codons within the active site of TS and obtain variants that are resistant to 5-fluorodeoxyuridine (5-FdUR). The resulting random library was selected for its ability to complement a TS-deficient Escher...

Predicting the toxicity of mixtures is an important current problem, and I'll talk about my personal view of the matter. I think that there are two broad classes of mixtures, one of which is the disposal mixtures: things that go into dump sites. These are highly variable from one site to another, since they depend on specific industrial operations, and it's difficult to extrapolate from site to...

Plastic behavioral adaptation to human activities can result in the enhancement and establishment of distinct behavioral types within a population. Such inter-individual behavioral variations, if unaccounted for, can lead to biases in our understanding of species’ feeding habits, movement pattern and habitat selection. We tracked the movements of 16 adult brown bears in a small and isolated pop...

Every 10 s, the antiproton-production-target will be bombarded by a pulse of 2×10 primary protons with an energy of 29 GeV (for an overview see [1]). This corresponds to a total beam power of about 10 kW. After passing of the first dipole primary protons and antiprotons are no longer in-line and the primaries have to be dumped. Behind the target a significant part of the beam power is carried b...

Drosophila mercatorum is a sexual species that can reproduce parthenogenetically in the laboratory. A previous study showed that a natural population of D. mercatorum inhabiting the Kamuela garbage dump on the Island of Hawaii could produce both viable parthenogenetic adults and self-sustaining parthenogenetic lines. The present study deals with a second screen for parthenogenesis and an isozym...

The paper presents a classification of fire extinguishing systems for dump trucks. principles fire-extinguishing operation are given according to the method actuation and purpose applied agents. Manufacturers mine trucks marked, technical characteristics their represented. As result research, it has been determined that main direction development is connected with class E fires, because increas...

The aim of this study was to evaluate the effect of comprehensive health risk protection behaviors, knowledge, attitudes, and practices among scavengers in open dump sites. A control group of 44 scavengers and an intervention group of 44 scavengers participated in this study. Interventions included the use of personal protective equipment, health protection training, and other measures. The ana...

The deoxythymidylate phosphohydrolase (dTMPase) induced by Bacillus subtilis bacteriophage PBS2 (whose DNA contains uracil instead of thymine) has been partially purified and shown to possess deoxyuridylate phosphohydrolase (dUMPase) activity. The similarities of induction period, pH dependence, heat and trypsin inactivation, sulfhydryl reagent and fluoride inhibition, metal ion effects, kineti...