Polyalthia longifolia (Sonn.) Thwaites is a traditional medicinal plant with diverse properties including kidney protection. The present investigation was aimed to assess the phytochemical content, antioxidant activity and evaluate nephroprotective of (PL) 70% ethanolic leaf extract against cisplatin gentamicin-induced toxicity on HEK293 cell line. Phytochemical analysis confirmed presence flav...

conclusions: despite the good in vitro bactericidal effect of amoxicillin on spiral forms of h. pylori, this antibiotic has little effect on induced coccoids that may develop after the inappropriate in vivo antibacterial treatment. hence, for successful therapy, it is essential not only to eradicate the spiral forms, but to eliminate the viable coccoids. background: helicobacter pylori (h. pylo...

Numerous procedures have been used to stain and clear nematode-infected plant tissues (7). Staining with acid fuchsin and destaining with lactophenol (4,7) has been the most widely used technique. More rap id means, of clearing and staining roots include autoclaving (1), exposure to saturated chloral hydrate (6), and root macera t ion after staining (3). However, extreme care is required for th...

Methotrexate, tetracycline, gentamicin, streptomycin, and penicillin inhibited the growth of L5178Y murine leukemia cells in culture with I(50) (concentration of drug that caused a 50% inhibition of growth at 72 h) values of 0.0028 mug/ml (6.2 x 10(-9) M), 7.9 mug/ml, 200 mug/ml, 1,700 mug/ml, and 3,000 mug/ml (5,000 U/ml), respectively. At concentrations achieved clinically or utilized in the ...

Elicitation with middle-viscous chitosan (30 mg/50 mL) significantly stimulated silymarin synthesis in Silybum marianum hairy root cultures. The root cultures established by infection with Agrobacterium rhizogenes AR15834 showed a potential for production of silymarin. Elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 mL) was used in order to imp...

This study aimed to determine the antibacterial activity and toxicity of methanol hexane extracts Halymenia durvillei red seaweed which were found abundantly in Kayangan island, South Sulawesi. The crude extract was tested against five gram-negative bacteria, namely Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Aeromonas hydrophila, Vibrio harveyi at a dose 200 g/disk. Extract on ...

Conflicting and complex forces are shaping the diffusion patterns of the Internet and e-commerce in South Asia. Drawing upon the literature on institutional theory, we explore the drivers and inhibitors of the Internet in South Asian countries. We examine the influence of the three pillars of institutions (Scott, 1995) on the digital world of South Asia. The paper discusses how regulatory, norm...

Sclerosants such as tetracycline (TCN) have often been used in the control of malignant pleural effusions. Although the resultant inflammatory response is probably important in the ensuing pleural fibrosis, the signals responsible for the cellular influx into the pleural space following TCN instillation are not well understood. This study, therefore, sought to determine whether the chemokines i...

Measurement of glutathione (GSH) and glutathione disulfide (GSSG) is a crucial tool to assess cellular redox state. Herein we report a direct approach to determine intracellular GSH based on a rapid chromatographic separation coupled with acidic potassium permanganate chemiluminescence detection, which was extended to GSSG by incorporating thiol blocking and disulfide bond reduction. Importantl...

The BAX system with automated PCR detection was compared with standard cultural procedures for the detection of naturally occurring and spiked Salmonella in 183 chicken carcass rinses and 90 chicken hot dogs. The automated assay procedure consists of overnight growth (16 to 18 h) of the sample in buffered peptone broth at 35 degrees C, transfer of the sample to lysis tubes, incubation and lysis...