The aim of this research is to evaluate a diagnostic model for uncovering an aflatoxicosis outbreak in sheep. The diagnostic model is based on differentiation between an aflatoxicosis suspected herd (ASH) and a control herd (CH) including, clinical findings, feed analysis for aflatoxin and other nutrients, hemato-chemical and biochemical profiles, histopathologic changes, and residual aflatoxin...

Little is known on the diversity and public health significance of Echinococcus species in livestock in Egypt. In this study, 37 individual hydatid cysts were collected from dromedary camels (n=28), sheep (n=7) and buffalos (n=2). DNA was extracted from protoscoleces/germinal layer of individual cysts and amplified by PCR targeting nuclear (actin II) and mitochondrial (COX1 and NAD1) genes. Dir...

Insect growth regulators (IGRs) are a class of new chemicals that interfere with maturation and reproduction in insects. Proposed hypotheses on the biochemical mechanism of action are presented herein. The environmental aspects as metabolism in soils, plants, insects, and animals suggest strongly that these chemicals undergo rapid degradation and metabolism to innocuous metabolites. The toxicol...

Sheep erythrocytes sensitized by incubation with the heat-labile enterotoxin (LT) of Escherichia coli are hemolyzed in the presence of anti-LT antiserum and complement. The Microtiter (Cooke Laboratory Products) technique was used to titrate anti-LT antibody in serum by this immune hemolysis reaction. Immune hemolysis was inhibited by preexposure of the anti-LT antiserum to soluble LT before ad...

This investigation aimed to evaluate the differential expression of HoxB7 and notch genes in different developmental stages of Echinococcus granulosus sensu stricto. The expression of HoxB7 gene was observed at all developmental stages. Nevertheless, significant fold differences in the expression level was documented in the juvenile worm with 3 or more proglottids, the germinal layer from infec...

The passive hemagglutination test with the sheep red blood cells coupled with Clostridium botulinum type E progenitor toxin is highly sensitive and specific to titrate type E botulinum antitoxin. The hemagglutination titers are proportional to the neutralization titers in sera obtained from immunized animals of different species, except for those taken at an early stage after the primary stimul...

Crude and excretory/secretory (E/S) antigens of Fasciola hepatica were subjected to SDS-PAGE and Western blot analysis in order to identify protein bands that would enable the specific and sensitive immunodiagnosis of sheep. Sera from 20 sheep with natural infections of F. hepatica, Dicrocoelium dendriticum, Cyst hydatid, Cysticercus tenuicollis, Trichostrongylidae, Paramphistomum spp., and Tri...

Retroviral-mediated gene transfer was used to insert a Neo R gene into fetal sheep hematopoietic cells obtained by exchange transfusion from lambs in utero. After gene transfer the cells were returned to the donor fetus. The lambs were examined after birth for the presence of a functioning Neo R gene. Of ten analyzable animals, six were positive for G418 resistant progenitor cells (CFU-Mix, CFU...

S: J. MYBURG.H & ]. DE V. DU TOIT. The influence of magnesium sulphate on the absorption, excretiOn and retentiOn of calcmm and phosphorus by sheep fed on phosphate supplemented rations. Onderstepoort]. vet. Res. 37 (1), 1-6 (1970). Eviden~e is l?ut [orward showing that continuous excessive intakes of magnesium sulphate by sheep, for example m dnnkmg water, may result in losses of calcium from ...

Methods Fifteen sheep had renal artery flow probes and renal vein cannula inserted. Ten sheep were given intravenous E. coli to induce sepsis and five acted as non-septic controls. Animals were managed for 48 hours with protocol guided ventilation, sedation, parenteral fluids and noradrenaline (NorA) infusion to maintain mean arterial pressure 75mmHg. Renal biopsies were taken from all animals ...