The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...

Gerbera spp., which is in the Asteraceae family, most widely cultivated cut flower ornamental plant Türkiye after carnation. Approximately 74% of Türkiye's production met from Antalya province. Virus diseases are a problem cultivation as they significantly reduce yield and quality. In this study, was carried out to detect some virus seen areas province, surveys were commercial 2021-2022 season....

Growing resistant cultivars is the best method of protecting the crops against Potato virus Y (PVY). There are a few sources of PVY resistance/tolerance in tobacco acquired through mass selection, X-ray induced mutagenesis and introgressions from wild Nicotiana species. Here, we compare major sources of PVY resistance/tolerance in inoculation tests using ten PVY isolates collected in Central Eu...

The aim of this study was to evaluate the occurrence of peony ring spot disease in the collections of foreign and Lithuanian origin peony cultivars and hybrids and to identify a causal agent of this viral disease. During surveys of ornamental plant collections grown in Lithuania, solitary instances of affected by peony ring spot disease plants have been detected in all collections surveyed. Dis...