نتایج جستجو برای: elisa and rt

تعداد نتایج: 16836501  

Journal: :iranian journal of virology 0
shirin farzadfar plant virus research department, iranian research institute of plant protection, tehran reza pourrahim plant virus research department, iranian research institute of plant protection, tehran mohammad torkian plant pathology department, varamin-pishva branch, islamic azad university, varamin mojdeh maleki plant pathology department, varamin-pishva branch, islamic azad university, varamin

background and aims: virus infections represent some of the most important diseases of lily, plants because of the devastating effects caused to the crops and the absence of effective treatments. a survey for virus diseases of lilies, revealed the occurrence of cucumber mosaic virus (cmv) in plants growing in tehran province, iran. materials and methods: during 2013, 50 lily samples with virus-...

Journal: :Asian Pacific Journal of Tropical Medicine 2021

Objective: To describe and molecularly characterize an outbreak of dengue virus (DENV) infection in Cajamarca, Andean region Peru.Methods: A total 359 serum samples from patients with acute febrile illness were assessed for the presence DENV via RT-PCR, ELISA NS1, IgM IgG Peru January 2017 to June 2017. The evaluation different diagnostic tests their applicability was performed.Results: Dengue ...

Journal: :Journal of infection in developing countries 2010
Mahmoud Mohamed Bahgat Mohamed Abd-Elhafez El-Far Ahmed Atef Mesalam Amany Abd-Elghany Ismaeil Ahmed Atef Ibrahim Hossam Eid Gewaid Amany Sayed Maghraby Mohamed Ahmed Ali Dina Nadeem Abd-Elshafy

BACKGROUND This work demonstrates successful propagation of HCV in HepG2 and human blood cells as well as viral shedding into their culture media. The influence of Schistosoma mansoni crude soluble egg antigens (SEA) on the rate of viral propagation in both mammalian cells was also monitored. METHODOLOGY HepG2 cells were inoculated with HCV viremic human sera and some wells were exposed to HC...

ژورنال: شیمی آلی و پلیمر 2017

شایع‌ترین ویروس تأثیرگذار بر کاهو در سراسر مزارع جهان، ویروس موزاییک کاهو (Lettuce mosaic virus; LMV) متعلق به خانواده Potyviridea و جنس Potyvirus است. روش‌های متعددی شامل آزمون‌های سرولوژیکی و مولکولی جهت تشخیص این ویروس وجود دارند، اما این روش‌ها زمان‌بر بوده و نیازمند ابزارهای پیچیده‌ای هستند. هدف از این پژوهش، کاهش زمان موردنیاز جهت تشخیص LMV با استفاده از روش تکثیر هم‌دمای وابسته به حلقه (...

Journal: :The Journal of infectious diseases 2007
Pierre Van Damme Carlo Giaquinto Melanie Maxwell Peter Todd Marie Van der Wielen

BACKGROUND Rotavirus gastroenteritis (RVGE) constitutes a significant burden of pediatric disease. Knowledge of currently cocirculating rotavirus genotypes is required to help guide immunization strategies. METHODS During the 2004-2005 RVGE season, a prospective, multicenter, observational study of RVGE was conducted in children <5 years of age seeking health care in primary care, emergency d...

Journal: :The Southeast Asian journal of tropical medicine and public health 2005
Philippe Buchy Van Luong Vo Khanh Toan Bui Thi Xuan Mai Trinh Philippe Glaziou Thi Thu Ha Le Viet Lo Le Trong Chien Bui

This study was designated to describe clinical and biological features of patients with a suspected diagnosis of dengue fever/dengue hemorrhagic fever during an outbreak in Central Vietnam. One hundred and twenty-five consecutive patients hospitalized at Khanh Hoa and Binh Thuan Provincial hospitals between November 2001 and January 2002 with a diagnosis of suspected dengue infection were inclu...

Journal: :Clinical and vaccine immunology : CVI 2006
V Balamurugan A Sen P Saravanan T J Rasool M P Yadav S K Bandyopadhyay R K Singh

We developed and characterized a stable Vero cell line constitutively expressing Peste des petits ruminants virus (PPRV) hemagglutinin (H) protein and assessed its potential use as diagnostic antigen in enzyme-linked immunosorbent assay (ELISA). PPRV H gene of the vaccine strain (Sungri-96) was amplified by reverse transcription (RT)-PCR, cloned into a eukaryotic expression vector (pTarget), an...

Journal: :حفاظت گیاهان 0
جعفرپور جعفرپور سبک خیز سبک خیز طبسی نژاد طبسی نژاد

abstract beet soilborne virus (bsbv) is a member species of the genus pomovirus with rigid rod particles and 3 positive single stranded rnas. the virus is transmitted by polymyxa betae and restricted to chenopodiaceae. a survey was conducted in 2005 for identification of bsbv in northern khorasan province. samples showing yellowing, elongated and upright petioles with narrow leaf lamina and hai...

2015
Fikriye Uras Burhanettin Küçük Özlem Bingöl Özakpınar Ahmet Muzaffer Demir

OBJECTIVE Growth arrest-specific 6 (Gas6) is a newly discovered vitamin K-dependent protein, which is a ligand for TAM receptors [Tyro3 (Sky), Axl, and Mer] from the tyrosine kinase family. Gas6 knockout mice were resistant to venous and arterial thrombosis. There are contradictory reports on the presence of Gas6 and its receptors in mouse platelets. The objective of this study was to investiga...

Journal: :Revue scientifique et technique 2004
S A Wani M A Bhat S M Ishaq M A Ashrafi

Rotavirus ribonucleic acid (RNA) was extracted from ten faecal samples of diarrhoeic calves positive for group A rotavirus by enzyme-linked immunosorbent assay (ELISA). A portion of the extracted RNA was run in polyacrylamide gel to determine the presence of rotaviral RNA and the rest subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) to generate the near full length VP7 gene...

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