electron transport system (ets) activity was found to be related to the initial inoculum size,culturability and viability of e. coli. in this experiment, ets activity of flexibacter chinensis was measuredby int-method under a variety of conditions such as starvation media with different amendments anddifferent temperatures. the relation between ets activity, cell culturability, and viability wa...

Signaling trough p53 is a major cellular stress response mechanism and increases upon nutrient stresses such as starvation. Here, we show in human hepatoma cell line that starvation leads to robust nuclear stabilization. Using BioID, determine the cytoplasmic interaction network within immediate-early dissociated from several metabolic enzymes kinase PAK2 for which direct binding with DNA-bindi...

Respiratory muscles are essential to alveolar ventilation. These muscles work against increased mechanical loads due to airflow limitation and geometrical changes of the thorax derived from pulmonary hyperinflation. Respiratory muscle fibres show several degrees of impairment in cellular and subcellular structures which, in many cases, are proportional to the severity of the disease and accompa...

Intestinal epithelial cells are tightly bound by tight junction proteins (TJP) which dynamic and sensitive to environmental stress. However, the role of endocytic pathway in regulation TJP abundance integrity during nutrient stress is poorly understood. Therefore, this study was conducted investigate starvation mechanism process. IPEC-J2 were subjected Krebs-Ringer bicarbonate buffer (KRB) TJP,...

Atox1, a copper transport protein, was recently identified as a copper-dependent suppressor of oxidative damage in yeast lacking superoxide dismutase. We have previously reported that Atox1 in the rat brain is primarily expressed in neurons, with the highest levels in distinct neuronal subtypes that are characterized by their high levels of metal, like copper, iron, and zinc. In this report, we...

Pyrvinium pamoate (PP) is an FDA-approved classical anthelmintic, but is now attracting particular attention as an anti-cancer drug after recent findings of its potent cytotoxicity against various cancer cell lines only during glucose starvation, as well as its anti-tumor activity against hypovascular pancreatic cancer cells transplanted in mice. The molecular mechanisms by which PP promotes su...

Thirty-two volunteers participated in a two-period crossover study in which ibuprofen was tested against an identical placebo for its effectiveness in reducing muscle soreness and damage after two bouts of downhill running. Subjective soreness, quadriceps isometric strength and isometric endurance time at 50 percent of maximum strength, serum activities of creatine kinase, lactate dehydrogenase...

The regulation of endurance training is usually based on the prescription of exercise intensity. Exercise duration, another important variable of training load, is rarely prescribed by individual measures and mostly set from experience. As the specific exercise duration for any intensity plays a substantial role regarding the different kind of cellular stressors, degree, and kind of fatigue as ...

[Purpose] The purpose of this study was to investigate the effects of 8 weeks of endurance exercise on the cardiac mitochondrial function of mice. [Subjects] Ten 129 SvJ/C57BL6 Male mice were used. The mice were randomly divided into an exercise group (n=5; mean ± SD weight, 27.4 ± 1.6 g) and a control (n=5; mean ± SD weight, 28.2 ± 1.1 g). The exercise mice ran on a motor driven treadmill 5 da...

Eukaryotes harbour a conserved signalling pathway, called General Amino Acid Control (GAAC) in Saccharomyces cerevisiae, for overcoming amino acid starvation. Upon starvation, the protein kinase Gcn2, which phosphorylates eukaryotic translation initiation factor eIF2α, becomes stimulated to trigger GAAC response. Genetic studies suggest that Yih1, is yeast homolog of mammalian IMPACT and binds ...