This research investigated the ability of on-farm pretreatments with acid, alkali, ozone or novel enzymes to improve enzymatic degradability of cellulose and hemicelluloses in biomass at the biorefinery. Two perennial grasses, switchgrass (Panicum virgatum) and reed canarygrass (Phalaris arundinacea L.), were direct-cut harvested, pretreated, and stored anaerobically for 30 d. Pretreated and un...