A cytolytic T lymphocyte (CTL) clone that lyses many HLA-A2 melanomas was derived from a population of tumor-infiltrating lymphocytes of an HLA-A2 melanoma patient. The gene coding for the antigen recognized by this CTL was identified by transfection of a cDNA library. It is the gene which has been reported to code for N-acetylglucosaminyltransferase V (GnT-V). Remarkably, the antigenic peptide...

When rat macrophages are activated by exposure to bacterial endotoxin they acquire the capacity to lyse sarcoma cells in tissue culture. Although showing no immunological specificity this lytic effect was only detectable on malignant cells; normal cells were unaffected. This tumor-selective lytic effect is mediated by a labile supernatant product.

Streptococcus pneumoniae is a major cause of invasive infection in young infants and older adults. There are currently 90 capsular serotypes identified and 23 serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19F, 19A, 20, 22F, 23F, and 33F) are responsible for about 90% of invasive disease. Among the more than 90 different S. pneumoniae serotypes, serotype 19A is g...

Abst ract. This pap er pr esents th e applica tion of signal flow gra phs to the det ermination of the exact values of gradient vecto r and hessian matrix for a linear system. It is shown all information about the gradient and hessian is cont ained in the or iginal and adjoint signa l flow graphs at different terminations. To det ermine the gra dient we have to perform two analyses , and t o ge...

Clot lysis methods are used extensively in fibrinolytic work both for research into the physiology of the fibrinolytic system and for assay and standardization of enzymes. The following is a description of a simple method for measuring partial clot lysis using cheap and easily available apparatus and reagents. The method was first described by Blix in 1962 and has been adapted to suit the appar...

Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, PhaxI, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm...

Tissue plasminogen activator (tPA) is an essential component of the proteolytic cascade that lyses blood clots. Various studies also suggest that tPA plays important roles in peripheral nerve regeneration. Here we show that disruption of tPA gene reduces macrophage migration after sciatic nerve injury in mice. Moreover, lack of tPA activity attenuates migrating ability of macrophages and affect...

Phaeocystis pouchetii virus (PpV01) infects and lyses the haptophyte Phaeocystis pouchetii (Hariot) Lagerheim and was first isolated from Norwegian coastal waters. We have used electron cryomicroscopy and three-dimensional image reconstruction methods to examine the native morphology of PpV01 at a resolution of 3 nm. The icosahedral capsid of PpV01 has a maximum diameter of 220 nm and is compos...

We have isolated a vesicular stomatitis virus (VSV)-specific CD4+ CD8- murine T-cell clone. This clone proliferates only in response to VSV and lyses infected tumor cells bearing class II major histocompatibility antigens in short-term chromium release assays. In addition, the cell has VSV antigens on its surface and is capable of killing uninfected tumor cells without major histocompatibility ...

The antioxidant potential of the ethanol extract of Vitex negundo L. was evaluated by using 1, 1diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide, total antioxidant capacity and erythrocyte (RBC) membrane stabilization assays. The extract showed significant activities in all antioxidant assays compared to the reference antioxidant ascorbic acid in a dose dependent manner. In DPPH and hydrogen...