In this study, we present the synthesis of chiral fragrance aldehydes, which was tackled by a combination chemo-catalysis and multi-enzymatic in vivo cascade reaction development highly versatile high-throughput assay for enzymatic reduction carboxylic acids. We investigated biocompatible metal-catalyzed preparation ? or ? substituted cinnamic acid derivatives were fed directly into biocatalyti...

Living systems rely on complex networks of chemical reactions to control the concentrations of molecules in space and time. Despite the enormous complexity in biological networks, it is possible to identify network motifs that lead to functional outputs such as bistability or oscillations. One of the greatest challenges in chemistry is the creation of such functionality from chemical reactions....

All chemical transformations pass through an unstable structure called the transition state, which is poised between the chemical structures of the substrates and products. The transi‐ tion states for chemical reactions are proposed to have lifetimes near 10-13 sec, the time for a single bond vibration. Thus, the transition state is the critical configuration of a reaction sys‐ tem situated at ...

Formulation of a truly advanced statistical theory of biochemical processes needs simple but realistic models of phenomena underlying microscopic dynamics of proteins. Many experiments performed in the 1980s have demonstrated that within the protein native state, apart from usual vibra-tional dynamics, a rich interconformational (activated) dynamics exists. The slowness of this dynamics makes a...

The ability to design artificial extracellular matrices as cell-instructive scaffolds has opened the door to technologies capable of studying the fate of cells in vitro and to guiding tissue repair in vivo. One main component of the design of artificial extracellular matrices is the incorporation of biochemical cues to guide cell phenotype and multicellular organization. The extracellular matri...

micro total analysis systems (μ-TAS),1 or labs-on-a-chip,2 have become of major interest, especially to analytical chemists due to its desirable characteristics, such as reduction in reagent consumption, required space, and analysis time. Taking these advantages, we have demonstrated many applications, including flow-injection analysis,3,4 solvent extraction,5–7 immunoassay,8,9 organic synthesi...

A novel fluorimetric method for the determination of peroxynitrite (ONOO-) using hemoglobin (Hb) as a catalyst is described. The method employs the reaction of ONOO with thiamine (TM), a colorless, non-fluorescent reagent in a glycine-NaCl-NaOH buffer solution (pH 12.7), to generate a highly fluorescent product, thiochrome (TC). The fluorescent product was monitored by fluorimetry. A linear cal...

The success of oligonucleotide ligation assays in probing specific sequences of DNA arises in large part from high enzymatic selectivity against base mismatches at the ligation junction. We describe here a study of the effect of mismatches on a new non-enzymatic, reagent-free method for ligation of oligonucleotides. In this approach, two oligonucleotides bound at adjacent sites on a complementa...

An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-...

Life is sustained by complex systems operating far from equilibrium and consisting of a multitude of enzymatic reaction networks. The operating principles of biology's regulatory networks are known, but the in vitro assembly of out-of-equilibrium enzymatic reaction networks has proved challenging, limiting the development of synthetic systems showing autonomous behaviour. Here, we present a str...