DNA ligases are essential both to in vivo replication, repair and recombination processes, and in vitro molecular biology protocols. Prior characterization of DNA ligases through gel shift assays has shown the presence of a nick site to be essential for tight binding between the enzyme and its dsDNA substrate, with no interaction evident on dsDNA lacking a nick. In the current study, we observe...

The effects of some antibiotics and metals on goat (Capra aegagrus hircus) kidney aldose reductase (AR) sorbitol dehydrogenase (SDH) activities were examined. For drugs that exhibit inhibitory effect, Ki constants calculated inhibition types determined by using Lineweaver-Burk curves. In our study, the highest showed ceftriaxone antibiotic in AR enzyme with an IC50 value 0.0274 mM. More potent ...