A comparison of haemagglutination inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) techniques for the detection of antibodies against Newcastle disease virus in sera from persons working in poultry farms and veterinary vaccine institutes and from the general population revealed that 22% more sera were positive by ELISA compared to HI. No samples were negative by ELISA but positive ...

An enzyme-linked immunosorbent assay has been developed to detect human C5a antigen. This ELISA methodology has been shown to be a highly sensitive technique capable of detecting C5a antigen concentrations below 10 ng/ml. The microELISA technique used in this study is specific for human C5a and C5a des arg (C5a antigen) but not for human C5. Conditions to establish sensitivity and specificity a...

There are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals EDCs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (VTG) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs especially...

The double-sandwich enzyme-linked immunosorbent assay has been compared with the indirect fluorescence assay for the detection of immunoglobulin M antibodies against Toxoplasma gondii in humans. Incubation times have been shortened, permitting the test to be completed within 2 h. The double-sandwich enzyme-linked immunosorbent assay is confirmed to be more sensitive and more specific than the i...

Herpes Simplex Virus Type 1 (HSV-1) is a ubiquitous pathogen. Other than known diseases, HSV-1 may have an important role in the pathogenesis of atopy by causing immortality of th2 cells. From June 1st to July 31st 2006, seventy five blood samples were collected from atopic children referred to the allergy clinic of the hospital. The bloods samples were used to detect HSV-1 IgG antibodies by En...

an enzyme-linked immunosorbent assay (elisa) for progesterone measurement in serum or plasma samples using penicillinase as label enzyme is reported. a c3 and c11 derivatives of progesterone were prepared and conjugated to bovin serum albumin (bsa). polyclonal antibody against these two immunogens were prepared in new zealand white rabbits. purified ig fractions of antibodies were immobilized o...

indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of h. contortus. plate elisa was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. indirect plate elisa was able to demonstrate...