CatalyCEST MRI can detect enzyme activity by monitoring the change in chemical exchange with water after a contrast agent is cleaved by an enzyme. Often these molecules use paramagnetic metals and are delivered with an additional non-responsive reference molecule. To improve this approach for molecular imaging, a single diamagnetic agent with enzyme-responsive and enzyme-unresponsive CEST signa...

Digitalization of fluorogenic enzymatic assays through the use of femtoliter chamber array technology is an emerging approach to realizing highly quantitative bioassays with single-molecule sensitivity. However, only a few digital fluorogenic enzyme assays have been reported, and the variations of the digital enzyme assays are basically limited to fluorescein- and resorufin-based fluorogenic as...

In the late 1960s and early 1970s ‘enzyme technology’ was viewed with the optimism and enthusiasm currently devoted to ‘biotechnology’. With one or two spectacularly successful exceptions, industry as a whole has not welcomed enzyme technology with open arms. Consideration of successful and less-successful processes indicates what industry wants and what it has been able to achieve; considerati...

the purpose of this study is to evaluate the impact of import of technology on iranian economy. we have used multi-sectoral and multi–regional computable general equilibrium gtap model. transfer of technology from one region to another is another factor effecting productivity. trade is one of the channels that speeds the transfer of technology. the effect of a ten percent productivity shock, in...

technology transmission has engaged not only developing countries but also many western research communities. foreign direct investment is a commonly used method to transfer the technology. since specific studies on factors affecting the transmission of technology through foreign direct investment has not been made in nanotechnology, the purpose of this study is to identify the issues affecting...

Enzyme-activity assays are used, for example, for screening enzyme inhibitors and activators to discover novel drug candidates. A homogeneous assay principle for hydrolyzing enzymes based on a double-labeled fluorogenic substrate is commonly employed and is suitable for high-throughput screening. This separation-free assay concept relies on the strong distance dependency of fluorescence resonan...

The generation of large mutant libraries for in vitro enzyme evolution presents the challenge of effectively screening libraries of 10(4)-10(7) mutants on the basis of simultaneously assaying their biocatalytic activity. In this review, we highlight the main steps involved in this process, describe the alternative approaches to address this challenge, survey the state-of-the-art technology and ...

Genome sequencing projects have provided researchers with a complete inventory of the predicted proteins produced by eukaryotic and prokaryotic organisms. Assignment of functions to these proteins represents one of the principal challenges for the field of proteomics. Activity-based protein profiling (ABPP) has emerged as a powerful chemical proteomic strategy to characterize enzyme function di...

As one of the emerging soil improvement techniques, microbial induced calcite precipitation (MICP) can bond soil grains together and improve the engineering properties of soil [1]. bacteria produces urease enzyme can catalyze urea to carbonate and result in calcite precipitation in elevated pH environment. The forms of calcite precipitation around the bacteria limit the availability of oxygen a...

The exploitation of enzymes for biotransformation reactions for the production of new and safer drug intermediates has been the focus of much research. While a number of enzymes are commercially available, their use in an industrial setting is often limited to reactions that are cost-effective and they are rarely investigated further. However, the development of miniaturized flow reactor techno...