We have identified a new benzopyran derivative, 3-(4-methoxy) phenyl-4-[[4-[2-(1-piperidinyl)ethoxy]phenyl]methyl]-2H-1-benzopyran-7-ol hydrochloride (CHF 4227), with improved in vivo estrogen agonist/antagonist effects. CHF 4227 binds with high affinity to the human estrogen receptor-alpha and -beta (dissociation constant K(i) = 0.017 and 0.099 nM, respectively). In immature rats, oral adminis...

This study aimed to investigate the male-to-female morphological and physiological transdifferentiation process in rainbow trout (Oncorhynchus mykiss) exposed to exogenous estrogens. The first objective was to elucidate whether trout develop intersex gonads under exposure to low levels of estrogen. To this end, the gonads of an all-male population of fry exposed chronically (from 60 to 136 days...

RATIONALE Trace levels of natural and synthetic steroid estrogens estrone (E1), 17β-estradiol (E2) and 17α-ethynyl estradiol (EE2) have been demonstrated to exert adverse effects in exposed organisms. E2 and EE2 have been proposed for inclusion in the Water Framework Directive (WFD) list of priority pollutants; however, the detection and accurate quantification of these compounds provide signif...

Functional in vitro and in vivo reporter gene assays have recently been developed for the rapid determination of exposure to (xeno)estrogens. The in vitro estrogen receptor (ER)-mediated chemically activated luciferase gene expression (ER-CALUX) assay uses T47D human breast cancer cells stably transfected with an ER-mediated luciferase gene construct. In the in vivo assay, transgenic zebrafish ...

The sheepshead minnow (Cyprinodon variegatus Lacépède), an estuarine fish species, was exposed to 17alpha-ethynylestradiol (EE2) at nominal test concentrations of 0.2, 2, 20, 200, 400, 800, 1,600, and 3,200 ng/L. Fish were exposed for up to 59 d, from subadult stages to sexual maturity, under flow-through conditions. The exposure period was followed by an evaluation of reproductive success and ...

A variety of anthropogenic compounds are capable of binding to the estrogen receptor (ER) of vertebrate species. Binding of these chemicals to the ER can interfere with homeostasis by altering normal gene expression patterns. The purpose of this study was to characterize the expression of 30 genes using a sheepshead minnow (Cyprinodon variegatus) cDNA macroarray. Many of the genes on the array ...

We calculate the contributions of weak magnetism and proton recoil order O(Ee2∕mN2)∼10−5, i.e. to next-to-next-to-leading in large nucleon mass expansion, neutron lifetime correlation coefficients beta decay, where Ee mN are electron energy mass, respectively. analyze electron-energy angular distribution for decay with a polarized neutron, an unpolarized proton. Together Wilkinson’s corrections...

ABSTRACT To determine the presence of emergent contaminants in aquatic environments and to evaluate responses dominant taxa benthic macrofauna, seven stations were sampled along three rivers that serve as public water suppliers sampling campaigns, Upper Iguassu Basin, Brazil. Concentrations ethinylestradiol, fenofibrate, ibuprofen triclosan detected sediment. correlate patterns distribution abu...

Hermansson, A. 2007. Effects on the reproductive system in domestic fowl (Gallus domesticus) after embryonic exposure to estrogenic substances. Doctor’s dissertation ISSN 1652-6880 ISBN 978-91-576-7319-0 Environmental pollutants with estrogenic activity have a potential to disrupt oestrogendependent developmental processes. Thus, the aim of this thesis was to investigated how embryonic exposure...