ABBREVIATIONS AA amino acid ANOVA one-way analysis of variance Asn 120 asparagine 120 BG blood glucose concentration BOR benzamidoxime reductase BZY benzydamine BZY N-oxide benzydamine N-oxide cDNA complementary DNA CYP cytochrome P450 cyt.b 5 cytochrome b 5 D50 50 mg/kg streptozotocin induced diabetic rats D70 70 mg/kg streptozotocin induced diabetic rats DAG diacyl-glycerol DIM 3,3'diindolylm...

The divalent cation chelator 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), often used to buffer physiological changes in cytosolic Ca(2+), also binds Zn(2+) with high affinity. In a recently published method (Lamboley et al. 2015. J. Gen. Physiol. http://dx.doi.org/10.1085/jgp.201411250), the absorbance shift of BAPTA at 292 nm was successfully used to determine the total ca...

We previously showed that adsorption of bacteriophages T4 and T5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. As these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal which must be transmitted between the two membranes. We show here that these two p...

Pemeriksaan bilirubin total merupakan salah satu pemeriksaan kimia klinik yang membutuhkan penanganan spesimen baik. Faktor dapat mempengaruhi hasil adalah cahaya, suhu penyimpanan dan waktu penyimpanan. Oleh karena itu harus segera dilakukan. menggunakan sampel plasma EDTA, dengan dikerjakan, dibandingkan serum, dibekukan terlebih dahulu selama 30 menit selanjutnya diperiksa sehingga mudah ter...

Diamine oxidase (DAO) is an enzyme synthesized primarily in the gastrointestinal mucosal cells. Serum levels of DAO have been used as an indicator of the integrity and/or functional mass of the intestinal mucosa. The enzyme is also produced by the placenta and is elevated in newborn serum. Previous radiometric methods for DAO used tritiated putrescine or cadaverine as substrate. A simple and ra...

OBJECTIVE In this study the mechanism of interactions between polyamidoamine (PAMAM) dendrimers and bovine insulin was examined. The insulin is a 51 amino acid peptide-hormone involved in the homeostasis of blood glucose levels. This molecule consists of two chains - A and B - linked by two disulphide bridges. As insulin contains four tyrosine residues it was possible to evaluate dendrimers eff...