OBJECTIVES The aim of this study was to evaluate the performance of a visual-tactile examination and a laser fluorescence device for detecting residual dentinal caries after carious dentin removal with bur excavation, hand excavation and chemomechanical excavation (Carisolv()]. METHODS Thirty extracted coronal caries primary second molars were used. The caries infected dentin has been removed...

Quantitative fluorescence microscopy is becoming an increasingly important tool in the study of cell biology. Fluorescence microscopy has long been used for qualitative characterizations of subcellular distributions of proteins, lipids, nucleic acids, and ions, but quantifying these distributions is complicated by a variety of optical, biological, and physical factors. Many factors that complic...

Early diagnosis and appropriate treatment play an important role in combating the mortality and morbidity caused by Tuberculosis and a rapid, reliable microscopy that can detect acid fast bacilli can be a very useful diagnostic modality. We have evaluated the performance of Light Emitting Diode Fluorescence Microscopy as an alternative to the existing conventional methods in the diagnosis of Pu...

A gene fusion system based on plasmid pBBR1MCS and the expression of green fluorescent protein was developed for Brucella suis, allowing isolation of constitutive and inducible genes. Bacteria containing promoter fusions of chromosomal DNA to gfp were visualized by fluorescence microscopy and examined by flow cytometry. Twelve clones containing gene fragments induced inside J774 murine macropha...

A new method in differentiation of chiral molecules is reported based on the fluorescence quenching of functionalized CdS quantum dots (CdS-QDs) as nanosensor by differing in the chirality of functionalization species. The chemically functionalized CdS-QDs with strong yellow emission were prepared using chiral L-cysteine (L-Cyst) and D-cysteine (D-Cyst) molecules. Then, the functionalized CdS-Q...