Immune responses can make protein therapeutics ineffective or even dangerous. We describe a general computational protein design method for reducing immunogenicity by eliminating known and predicted T-cell epitopes and maximizing the content of human peptide sequences without disrupting protein structure and function. We show that the method recapitulates previous experimental results on immuno...

bacteria usually cause disease in the gastrointestinal tract by colonization and growth in the gastrointestinal tract, where the micro organisms may invade the tissues of host or secretion of an exotoxin that is performed in food and then ingested by the host, examples of second group include: staphylococcal, bacillus cereus, clostridial (c.botulinum and c.perfringens type a) toxin. in this stu...

Pseudomonas exotoxin A (PE) is one of the most potent cytotoxic agents produced by Pseudomonas aeruginosa. In this study, we examined the possibility of using PE with a deletion of 38 carboxyl-terminal amino acid residues, designated PE(Delta576-613), for active immunization against PE-mediated disease. We first examined the toxic effects of PE and PE(Delta576-613) on 5- and 9-week-old ICR mice...

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