REPORTS Recombinant DNA: Examples ofPresent-Day Research:J.Abelson. Charon Phages: Safer Derivatives ofBacteriophage Lambda forDNA Cloning: F. R. Blattner et al. . Recombinational Switch for Gene Expression: J. Zieg et al. Gene Cloning and Containment Properties of Plasmid Col El and Its Derivatives: K. A. Armstrong, V. Hershfield, D. R. Helinski. EK2 Derivatives ofBacteriophage Lambda Useful i...

Antimicrobial activity of certain species Lactobacillus bacteria were known to produce a type bacteriocin called Plantaricin (Pln). In this study, the PlnF (plnF) gene (666 bp) encoding plantaricin was synthetically constructed based on sequence predefined and valuable amino acid compositions subcloned into pET-28 (+) expression vector. Thereafter transformation recombinant vector BL21 (DE3) co...

BACKGROUND RNA interference (RNAi) is a conserved gene silencing mechanism mediated by small inhibitory microRNAs (miRNAs).Promoter-driven miRNA expression vectors have emerged as important tools for delivering natural or artificially designed miRNAs to eukaryotic cells and organisms. Such systems can be used to query the normal or pathogenic functions of natural miRNAs or messenger RNAs, or to...

Background and Aims: Influenza (flu) is a respiratory infection in mammals and birds. It is caused by an RNA virus in the family Orthomyxoviridae. The virus is divided into three main types. Influenza virus type A is found in a wide variety of bird and mammal species and can undergo major shifts in immunological properties. Hemagglutinin (HA) is an important influenza virus surface antigen that...

We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that...