2.1.1 Aqueous extracts preparation The dried form of plant was mixed with sterile distilled water in a ration of 1:20 (100 g in 1 L solvent), and was stirred and heated for about 4 hours. After cooling, the extract was filtered by using Whatman No.1 filter paper. The filtrate was collected and frozen in ice cube container. The frozen ice cube was freeze-dried (i.e. lyophilisation) to obtain con...

Viscum album L. is a parasitic plant that grows on various trees, in our days, mistletoe extracts are widely used in addition to the treatment of cancer. The aim of the present study is to asses the efficiency of mistletoe extract in combination therapy with doxorubicin chloride, a widely used chemotherapeutic agent. The Viscum album (plant raised from the apple tree) was harvested from Cluj ar...

Photosynthetic effects of the leaves extracts from Ipomoea cairica on Parthenium hysterophorus L were studied. Ipomoea cairica dried leaf powder (20g) was dissolved in distilled water (200ml) for foliar application. The seedlings were maintained under natural condition in Insect free net house. The experiment was carried out to analyze the effects of Ipomoea cairica leaves extract by measuring ...

The first paper of this series (Roy, 1953) reported the presence of two distinct fractions exhibiting sulphatase activity in an aqueous extract of an acetone powder of ox liver, and gave preliminary data on the properties of the two crude enzymes, which were separated by fractional precipitation with acetone. The present paper describes the further purification and properties of fraction A, now...

Extracts of acetone powders of Flavobacterium heparinum which have been adapted to heparin catalyze a rather extensive degradation of this mucopolysaccharide. This ‘Lheparinase” activity is due to the presence in the extract of a sulfamidase, a sulfesterase, and at least one glycosidase (1, 2). Such extracts also catalyze the hydrolysis of hyaluronic acid and chondroitin sulfate. This paper com...

Anticentromere antibodies (ACA) Discrete speckled pattern on Hep-2 cells in interphase (8, 9). Anti-DNA topoisomerase I At a serum dilution of 1:40 by the Outcherlony double immunodiffusion method using as the antigen source a freshly prepared saline extract of lyophilized rabbit thymus acetone powder at a concentration of 20-40 mg protein/ml and a reference serum for comparison. Other procedur...

The crude methanolic extract of Averrhoa bilimbi Linn. (Oxalidiaceae) fruits and its different fractions have been investigated for the evaluation of in vitro cytotoxic potential. The dried and powder fruits were extracted with methanol at room temperature and the concentrated methanolic extract was fractionated by the modified Kupchan partitioning method to provide pet-ether, carbon tetrachlor...

Rhizomes of Boesenbergia pandurata (Roxb.) Schlecht have been reported to contain active compounds with anticancer properties. This research was carried out to examine anti-proliferative and apoptotic induction against HeLa and Vero cells-line. Dried powder of B. pandurata rhizomes was extracted by a maceration method using 90% ethanol. Cytotoxic assays to determine IC50 and anti-proliferative ...