The purpose of the present study is to survey the extraction conditions and explore the antioxidant potential of the wild herb Cistus incanus, which is non-traditional in Bulgarian ethnomedicine and widespread in the Strandja Mountain. The influence of the extraction time (0-500 min) and solvent composition (0-50% ethanol in water) on the polyphenols, flavonoid yields and on the antioxidant cap...

PREMISE OF THE STUDY An efficient, effective DNA extraction method is necessary for comprehensive analysis of plant genomes. This study analyzed the quality of DNA obtained using paper FTA cards prepared directly in the field when compared to the more traditional cetyltrimethylammonium bromide (CTAB)-based extraction methods from silica-dried samples. METHODS DNA was extracted using FTA cards...

The first step in biomarkers discovery is to identify the best protocols for their purification and analysis. This issue is critical when considering peripheral blood samples (plasma and serum) that are clinically interesting but meet several methodological problems, mainly complexity and low biomarker concentration. Analysis of small molecules, such as circulating microRNAs, should overcome th...

With the rapid development of molecular biology and the life sciences, magnetic extraction is a simple, automatic, and highly efficient method for separating biological molecules, performing immunoassays, and other applications. Human blood is an ideal source of human genomic DNA. Extracting genomic DNA by traditional methods is time-consuming, and phenol and chloroform are toxic reagents that ...

AB Archival samples represent a significant potential for genetic studies, particularly in severe diseases with risk of lethal outcome, such as in cancer. In this pilot study, we aimed to evaluate the usability of archival bone marrow smears and biopsies for DNA extraction and purification, whole genome amplification (WGA), multiple marker analysis including 10 short tandem repeats, and finally...

Dispersive Liquid-Liquid Microextraction / Thermal Lens Microscopy (DLLME/TLM) was developed as a new combination method for preconcentration and determination of Cd. Thermal Lens Microscopy is suitable for the determination of analyte after DLLME because of the low volume of the remained phase after DLLME and increasing of the enhancement factor for the nonpolar organic solvents. Some effe...

Photoreactions of the fourth rhodopsin-like pigment, phoborhodopsin (pR480), of Hulobacrerium halobium were studied by low-temperature spectrophotometry. Upon irradiation of pR480 at -80 as well as 170°C with 436 nm light, a new intermediate having a difference absorption maximum at about 520 nm was produced. We designate this intermediate ‘P520’. Prolonged irradiation caused the formation of a...