Desertomycin was isolated from Streptomyces macronensis Dietz sp. nov. UC 8271. Extensive spectroscopic work led us to place desertomycin in the macrocyclic lactone family which contains monazomycin, scopafungin, primycin, azalomycin F4a and niphithricins A and B. The apparent molecular formula was determined by fast atom bombardment mass spectroscopy to be C57H109NO24 (MW = 1,191). Mild acid h...

A strain of Bacillus polymyxa produced a new peptide antibiotic complex, named LI-F, composed of more than ten components. The components, antibiotics LI-F03, F04, F05, F07, and F08 were isolated from the complex by reversed phase HPLC. They are active against fungi, yeasts, and Gram-positive bacteria. The fast atom bombardment mass spectra revealed that the individual isolated antibiotics are ...

The structures of two new, naturally occurring cytotoxic depsipeptides, tamandarins A and B (1 and 2), are presented. The tamandarins were isolated from an unidentified Brazilian marine ascidian of the family Didemnidae. The structures of the new cytotoxins were assigned by interpretation of FABMS data and by extensive 2D NMR analyses. The absolute configurations of the tamandarins were assigne...

Polar and primary metabolites of cyclosporin A (CsA) have successfully been isolated by a novel separation protocol. An efficient, easy-to-scale-up chromatographic adsorption/desorption operation recovers polar and primary CsA metabolite pools from large volumes of urine; purified CsA metabolites are subsequently obtained by high-resolution preparative elution chromatography of the semipurified...

Takagi-Sugeno type fuzzy models are widely used for model-based control and model-based fault diagnosis. They provide high accuracy with relatively small and easy to interpret models. The problem that we address in this paper is that data driven identification of such fuzzy models is computationally costly. Whereas most identification algorithms for Takagi-Sugeno models restrict the model’s gen...

The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp). Combining unfolding by a temperature gradient in a thermal cycler with simultaneous proteolytic cleavage of the unfolded state, we probed stability of single domains in lysates. ...

The Fast Chirp Transform (FCT) provides a powerful formalism for the detection of signals with variable frequency, in much the same way that the Fourier transform provides a formalism for the detection of constant frequency signals. The FCT algorithm has significant applications in the detection of gravitational waves, particularly the gravitational wave signal produced by an inspiralling compa...

We have developed a multi-wavelength Fast Temperature Readout (FasTeR) spectrometer to capture a sample's transient temperature fluctuations, and reduce uncertainties in melting temperature determination. Without sacrificing accuracy, FasTeR features a fast readout rate (about 100 Hz), high sensitivity, large dynamic range, and a well-constrained focus. Complimenting a charge-coupled device spe...

The V-FASTR experiment on the Very Long Baseline Array was designed to detect dispersed pulses of milliseconds in duration, such as fast radio bursts (FRBs). We use all V-FASTR data through 2015 February to report V-FASTR’s upper limits on the rates of FRBs, and compare these with rederived rates from Parkes FRB detection experiments. V-FASTR’s operation at l = 20 cm allows direct comparison wi...

Calcium absorption was measured in ten male volunteers from skimmed milk, Ca-enriched skimmed milk or watercress (Nasturtium officinale) soup. The foods were labelled extrinsically with 30 mg 44Ca. Shortly after consuming the labelled meal, each subject was given an intravenous injection of 3 mg 42Ca. Fractional absorption from the oral dose was determined from plasma and urine samples collecte...