Skin surface lipid samples were collected by the hexane sponge technique from the foreheads of 180 male and 131 female Saudi subjects living in the Jeddah-Makkah area (summer temperatures up to 47 degrees C). The lipid samples were analyzed by densitometric thin layer chromatography. Seven major lipid classes were determined. The percentages for squalene, cholesterol esters, wax esters, triglyc...

Carboxylic acid esters react with hydroxylamine in alkaline solution to form hydroxamic acids. These acids produce a red to violet reaction with ferric chloride which has been utilized by Feigl (1949) for the microchemical detection of carboxylic acid derivatives. Lipmann and Tuttle (1945) developed a method for the quantitative assay of acylphosphates by allowing hydroxylamine to react with th...

A rapid and simple method for separation of serum lipid classes for fatty acid analysis with a single aminopropyl solid phase glass column is described. The recoveries of cholesteryl esters, triglycerides, free fatty acids, and phospholipids were all at least 98%. Coefficients of variation less than 10% were obtained for absolute and relative amounts of most individual fatty acids analyzed afte...

The aim of the present study was to assess the effect of slaughter and carcass weight, age at slaughter and sex on the fatty acid profile of the Greek Boutsiko sheep breed. Twenty lambs (ten females and ten males) were examined. The lambs were slaughtered at the age of 48 days. Carcasses were chopped in nine specific cuts and a random sample of 100 g was taken from each cut, with a total of 180...

A reduction of the cost enzymatic biodiesel is crucial to make it competitive with known technologies. single-step process for conversion high free fatty acid oils into ethyl esters using a low-cost lipase was developed.

The enzymatic indirect method for simultaneous determinations of 3-chloro-1, 2-propanediol fatty acid esters (3-MCPD-Es) and glycidyl fatty acid esters (Gly-Es) make use of lipase from Candida cylindracea (previously referred to as C. rugosa). Because of low substrate specificity of the lipase for esters of polyunsaturated fatty acids (PUFA), such as docosahexaenoic acid (DHA) and eicosapentaen...

Nonesterified fatty acids accumulate at sites of tissue injury and necrosis. In cardiac tissue the concentrations of oleic acid, arachidonic acid, leukotrienes, and other fatty acids increase greatly during ischemia due to receptor or nonreceptor-mediated activation of phospholipases and/or diminished reacylation. In ischemic myocardium, the time course of increase in fatty acids and tissue cal...

the aim followed in this study was to make a comparison of the composition of fatty acids available in the sperm lipids of cattle, sheep and goat. semen samples were collected from 28 holestein cows, 12 chal rams and 7 mahabadi goats. after washing out and removal of the seminal plasma, total lipids of the sperm were extracted and fatty acids trans-methylated. the fatty acid methyl esters were ...

A series of isopropyl fatty esters having different chain length (C(13)-C(23)) were synthesized and formulated in lipid nanoparticles based on Precirol ATO to evaluate their effect on the physicochemical properties of these latter. Moreover, drug loading and skin permeation of Econazole nitrate, chosen as a lipophilic model drug, were evaluated as well. The obtained nanosystems, prepared by hig...

Proteomic analysis of mouse kidney peroxisomes resulted in the identification of a novel nudix hydrolase designated RP2p, which is encoded by the D7RP2e gene. RP2p consists of 357 amino acids and contains two conserved domains: a nudix hydrolase domain and a CoA-binding domain. In addition, a PTS (peroxisomal targeting signal) type 1 (Ala-His-Leu) was found at the C-terminus. Analysis of the en...