The functional properties of the recombinant C-terminal dimerization domain of the Pseudomonas aeruginosa Fur (ferric uptake regulator) protein expressed in and purified from Escherichia coli have been evaluated. Sedimentation velocity measurements demonstrate that this domain is dimeric, and the UV CD spectrum is consistent with a secondary structure similar to that observed for the correspond...

Sorghum/millet-based beverages, Obiolor and Pito, were evaluated for their nutritional and antioxidant dispositions. Analyzed Obiolor and Pito contained 96% and 97% moisture; 7.8% and 3.7% crude protein; 8.9% and 5.6% available carbohydrate; 0.39% and 0.31% crude fat; 0.3% and 0.2% crude fiber; 2.4% and 1.5% ash; and 459.3 and 164 kJ/g energy value, respectively. Obiolor and Pito (1.0 mL) scave...

Particle size, pH and Fe(III) ions affect the process of bioleaching of copper from chalcopyrite ores. In the study presented herein a copper sulfide ore was subjected to bioleaching process using a mixed microbial consortium obtained from a biotrickling filter treating high loads of H2S at different mineral particle size, distinct medium pH and various additional Fe(III) ion concentrations as ...

The present work is carried out to evaluate the antioxidant potential of fractions from ethanolic extract of Alstonia scholaris Linn. (Apocynaceae) using various in vitro tests including 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging, metal ion chelating, hydrogen peroxide scavenging, superoxide anion radical scavenging, and ferric thiocyanate reducing ability. Dichloromethane an...

Three simple, accurate and economical spectrophotometric methods in ultraviolet and visible region were developed for the determination of cefprozil in bulk and dosage forms have been described. The method A is based on the reduction of ferric ion into ferrous ion by the mentioned drug in the presence of 1,10-phenanthroline to form a highly stable orange red colored ferrion complex measured at ...

The ferric uptake regulator (Fur) plays a critical role in the transcriptional regulation of iron metabolism. However, the full regulatory potential of Fur remains undefined. Here we comprehensively reconstruct the Fur transcriptional regulatory network in Escherichia coli K-12 MG1655 in response to iron availability using genome-wide measurements. Integrative data analysis reveals that a total...