The chemical modification of two new double-headed protease inhibitors from black-eyed peas, a trypsin-chymotrypsin inhibitor (BEPCI) and a trypsin inhibitor (BEPTI) with dansyl chloride was investigated under various conditions. The NH,-terminal serine of both BEPCI and BEPTI, the 4 lysyl residues of BEPCI, and 4 of the 5 lysyl residues of BEPTI, could not be dansylated in the absence of urea....

We describe a new approach to calculating photon trajectories and gravitational lensing effects in the strong gravitational field of the Kerr black hole. These techniques are applied to explore both the imaging and spectral properties of photons that perform multiple orbits of the central mass before escaping to infinity. Viewed at large inclinations, these higher order photons contribute ∼ 20%...

The ability to predict the effects of extreme climatic variables on livestock is important in terms of welfare and performance. An index combining temperature and humidity (THI) has been used for more than 4 decades to assess heat stress in cattle. However, the THI does not include important climatic variables such as solar load and wind speed (WS, m/s). Likewise, it does not include management...

OBJECTIVE To identify the sensitivity of several readily available diagnostic tests for onychomycosis. DESIGN Cross-sectional study. SETTING Dermatology and podiatry departments at a teaching hospital. PATIENTS Sixty-three adult men and women with a clinical examination highly suggestive of onychomycosis. MAIN OUTCOME MEASURES Sensitivity of each test and of several test combinations. ...

As water resources become further stressed due to increasing levels of societal demand, understanding the effect of climate and landuse change on various components of the water cycle is of strategic importance. In this study we used a previously developed hydrologic model of the Black Sea Catchment (BSC) to assess the impact of potential climate and landuse changes on the fresh water availabil...

Introduction: The presence of garnet in the lunar mantle has been debated from many years (e.g., [1-3]). Data from the Apollo seismic network were unable to unambiguously resolve a garnet signature in the lower mantle. For example, higher seismic velocities in the lunar mantle (>500 km) could be indicative of an increased proportion of Mg-rich olivine [2,4], or be due to the presence of garnet ...

Several recent studies show that the lungs infected with Pseudomonas aeruginosa are often co-colonised by oral bacteria including black-pigmenting anaerobic (BPA) Porphyromonas species. The BPAs have an absolute haem requirement and their presence in the infected lung indicates that sufficient haem, a virulence up-regulator in BPAs, must be present to support growth. Haemoglobin from micro-blee...

During a study of nucleolar organiser regions, a modified silver stain was found to be a sensitive marker for the iron in ringed sideroblasts, more so than Perls's stain when the marrow iron stores were low. To enhance the usefulness of the silver stain, a combined silver Perls method was developed. This stains the ringed sideroblast iron black and haemosiderin blue, thus rendering the detectio...

Giardia intestinalis is a parasitic protozoan that causes diarrhea and abdominal pain in humans. Studies of the Giardia genotypes are thought to be important for understanding their infection routes and prevalence. However, few have reported pathogen genotyping in human giardiasis cases in Japan. In this study, we genotyped G. intestinalis by using DNA extracted from chlorazol black E-stained f...

The study examines the ability of three volatility-forecasting models to estimate the term structure of implied volatilities. The tests are performed on equity Warrants listed on the JSE with the three measures being the Generalized Autoregressive Conditional Heteroscedicity (GARCH), the exponential GARCH (EGARCH) and the Exponentially Weighted Moving Average (EWMA). The Black-Scholes implied v...