Yeasts can be enriched with microelements, including iron; however, special physicochemical conditions are required to formulate a culture media that promotes both yeast growth and iron uptake. Different iron sources do not affect biomass formation; however, considering efficacy, cost, stability, and compatibility with Saccharomyces cerevisiae metabolism, ferrous sulphate is recommended.

Iron supplements such as ferrous sulfate tablets are usually used to treat iron-deficiency anemia in some elderly patients with primary neurologic disorders or decreased gag reflexes due to stroke, senile dementia, or parkinsonism. While the aspiration of ferrous sulfate is rarely reported, it is a potentially life-threatening condition that can lead to airway necrosis and bronchial stenosis. A...

Hydrogen peroxide reacts with ferrous Ascaris hemoglobin and converts it to oxyhemoglobin in a sequence of two reactions. The first is the reaction of ferrous Ascaris hemoglobin with hydrogen peroxide to form a stable product; the second is the reaction of this product with hydrogen peroxide to form oxyhemoglobin. Both reactions follow second order kinetics, since they are first order with resp...

Huntington's disease (HD) is a progressive neurodegenerative disorder caused by a polyglutamine-encoding CAG expansion in the huntingtin gene. Iron accumulates in the brains of HD patients and mouse disease models. However, the cellular and subcellular sites of iron accumulation, as well as significance to disease progression are not well understood. We used independent approaches to investigat...

R ECENTLY a new reagent was proposed for the determination of cholesterol (1, 2). This reagent was purported to be saturated ferrous sulfate in glacial acetic acid. Since it has been reported that ferrous sulfate is qualitatively insoluble in anhydrous acetic acid (3, 4) and that ferrous iron is “subject to pronounced air oxidation in glacial acetic acid” (5), it was felt that several factors s...

The effects of cholesterol and Tween 80 on the physical stability of empty liposomes were investigated. Results showed that the physical stability of liposomes, including electrostatic and steric stability, was improved by addition of cholesterol and Tween 80. Liposomes prepared by different methods, thin-film hydration, thin-film sonication, reverse-phase evaporation and freeze-thawing, were t...

UNLABELLED Feo is the major ferrous iron transport system in prokaryotes. Despite having been discovered over 25 years ago and found to be widely distributed among bacteria, Feo is poorly understood, as its structure and mechanism of iron transport have not been determined. The feo operon in Vibrio cholerae is made up of three genes, encoding the FeoA, FeoB, and FeoC proteins, which are all req...

Haem binding to human serum albumin (HSA) endows the protein with peculiar spectroscopic properties. Here, the effect of ibuprofen and warfarin on the spectroscopic properties of ferric haem-human serum albumin (ferric HSA-haem) and of ferrous nitrosylated haem-human serum albumin (ferrous HSA-haem-NO) is reported. Ferric HSA-haem is hexa-coordinated, the haem-iron atom being bonded to His105 a...

The immobilization of Acidithiobacillus ferrooxidans cells on ceramic beads as carrier is described. The effects of ferrous ion concentration and dilution on the kinetics of ferrous ion oxidation in a packed-bed bioreactor were studied. In a medium containing 13.91 g of ferrous ion per litre, the fastest oxidation rate was 4.21 g L–1 at a dilution rate of 0.8 h–1. The corresponding conversion w...

The absorption of metals from the nasal cavity to the blood and the brain initiates an important route of occupational exposures leading to health risks. Divalent metal transporter-1 (DMT1) plays a significant role in the absorption of intranasally instilled manganese, but whether iron uptake would be mediated by the same pathway is unknown. In iron-deficient rats, blood (59)Fe levels after int...