BACKGROUND The sensitivity for identification of malignant cells in conventional fine-needle aspiration biopsy (FNAB) investigation is about 80%. This percentage is dependent on the number of examined cells, type of breast cancer, and experience of the examiner. The aim of our study was to estimate the supporting value of image DNA cytometry of FNAB of the breast, and do so by using different s...

This paper documents a multi-level microcosm system: Dreissena-ciliates-bacteria-viruses. In the first comprehensive investigation of endocytobionts present in the cytoplasm of endosymbiotic ciliates from mussels, bacteria are described from Conchophthirus acuminatus and an undescribed Ophryoglena sp. species which are, respectively, commensal and parasitic in the freshwater bivalve Dreissena p...

Background: The main goals of our study were as follows: (1) to investigate whether the methanolic extract of the new cultivar (Gürarslan) of Trigonella foenum graecum L. seeds exhibit antiproliferative effects on the HeLa human cervix carcinoma, Mat-LyLu rat prostate carcinoma and 3T3 embryonic mouse fibroblast cell lines, (2) to determine how mitosis and DNA synthesis are affected in cancer c...

" siderocyte " to describe erythrocytes containing small granules readily demonstrable by means of Perls's (Prussian blue) reaction. He found these cells in small numbers in the blood of normal rat, mouse, and human embryos (Gruneberg, 1941a and b), and later (Gruneberg, 1942) in large numbers in the blood of mice suffering from congenital anaemia. Doniach, Gruneberg, and Pearson (1943) describ...

AIM To compare ploidy measurements obtained on tissue sections of selected low and high grade squamous intraepithelial lesions containing oncogenic HPV (types 16, 18 or 33) detected by in situ hybridisation (ISH) or PCR. METHODS DNA ploidy was assessed by image cytometry after Feulgen staining of contiguous serial sections of eight lesions exhibiting atypical squamous cells or squamous atypia...

In tumour cell lines, the resistance of cancer cells to a variety of structurally unrelated chemotherapeutic drugs is termed multidrug-resistance or MDR. We reported previously [6] that MDR leukemic cells displayed nuclear texture changes, as assessed by image cytometry. The nature of these changes remained uncertain but they could be associated with alterations of the nuclear matrix which coul...

Flow cytometric measurements of nuclear DNA content were performed using ethidium bromide as the DNA stain (internal standard, Hordeum vulgare 'Ditta', 1C = 5.063 pg) in 25 samples belonging to nine diploid species and four varieties of Capsicum: C. chacoense, C. parvifolium, C. frutescens, C. chinense, C. annuum var. annuum, C. baccatum var. baccatum, C. baccatum var. pendulum, C. baccatum var...

Recent advances in computer technology have been made and image analysis (IA) has been introduced into pathological fields. The present study aimed to investigate the utility of IA for the evaluation of nuclear features and staining of immunohistochemistry (IHC) for Ki-67, p53 and GATA-binding protein 3 (GATA-3) in urothelial carcinoma tissue samples. A total of 49 cases of urothelial carcinoma...